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    NIFK nucleolar protein interacting with the FHA domain of MKI67 [ Homo sapiens (human) ]

    Gene ID: 84365, updated on 5-May-2024

    Summary

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    Official Symbol
    NIFKprovided by HGNC
    Official Full Name
    nucleolar protein interacting with the FHA domain of MKI67provided by HGNC
    Primary source
    HGNC:HGNC:17838
    See related
    Ensembl:ENSG00000155438 MIM:611970; AllianceGenome:HGNC:17838
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    Nopp34; MKI67IP
    Summary
    This gene encodes a protein that interacts with the forkhead-associated domain of the Ki-67 antigen. The encoded protein may bind RNA and may play a role in mitosis and cell cycle progression. Multiple pseudogenes exist on chromosomes 5, 10, 12, 15, and 19.[provided by RefSeq, Jan 2009]
    Expression
    Ubiquitous expression in lymph node (RPKM 19.8), appendix (RPKM 17.9) and 25 other tissues See more
    Orthologs
    mouse all
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    Genomic context

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    Location:
    2q14.3
    Exon count:
    7
    Annotation release Status Assembly Chr Location
    RS_2023_10 current GRCh38.p14 (GCF_000001405.40) 2 NC_000002.12 (121726945..121736875, complement)
    RS_2023_10 current T2T-CHM13v2.0 (GCF_009914755.1) 2 NC_060926.1 (122162220..122172150, complement)
    105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) 2 NC_000002.11 (122484521..122494451, complement)

    Chromosome 2 - NC_000002.12Genomic Context describing neighboring genes Neighboring gene NIFK antisense RNA 1 Neighboring gene uncharacterized LOC124906186 Neighboring gene nucleophosmin 1 pseudogene 32 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 16471 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 11923 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 16472 Neighboring gene translin Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr2:122533206-122534180 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr2:122534181-122535155 Neighboring gene uncharacterized LOC105373590 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr2:122547721-122548382

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: HPA RNA-seq normal tissues
    • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
    • BioProject: PRJEB4337
    • Publication: PMID 24309898
    • Analysis date: Wed Apr 4 07:08:55 2018

    Bibliography

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    Related articles in PubMed

    1. The RNA recognition motif of NIFK is required for rRNA maturation during cell cycle progression. Pan WA, et al. RNA Biol, 2015. PMID 25826659, Free PMC Article
    2. Direct observations of shifts in the β-sheet register of a protein-peptide complex using explicit solvent simulations. Panteva MT, et al. Biophys J, 2011 May 4. PMID 21539773, Free PMC Article
    3. Sequential phosphorylation and multisite interactions characterize specific target recognition by the FHA domain of Ki67. Byeon IJ, et al. Nat Struct Mol Biol, 2005 Nov. PMID 16244663
    4. Structure of human Ki67 FHA domain and its binding to a phosphoprotein fragment from hNIFK reveal unique recognition sites and new views to the structural basis of FHA domain functions. Li H, et al. J Mol Biol, 2004 Jan 2. PMID 14659764
    5. The nucleolar protein NIFK promotes cancer progression via CK1α/β-catenin in metastasis and Ki-67-dependent cell proliferation. Lin TC, et al. Elife, 2016 Mar 17. PMID 26984280, Free PMC Article

    See all (102) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. NIFK as a potential prognostic biomarker in colorectal cancer correlating with immune infiltrates.
      Title: NIFK as a potential prognostic biomarker in colorectal cancer correlating with immune infiltrates.
    2. NIFK is required for lung cancer progression via the RUNX1-dependent CK1alpha repression, which activates TCF4/beta-catenin signaling in metastasis and the Ki-67-dependent regulation in cell proliferation.
      Title: The nucleolar protein NIFK promotes cancer progression via CK1α/β-catenin in metastasis and Ki-67-dependent cell proliferation.
    3. The RNA recognition motif of NIFK is required for rRNA maturation during cell cycle progression.
      Title: The RNA recognition motif of NIFK is required for rRNA maturation during cell cycle progression.
    4. Potential encounter complexes of the Ki67FHA receptor and hNIFK peptide are misregistered states of the beta-sheet.
      Title: Direct observations of shifts in the β-sheet register of a protein-peptide complex using explicit solvent simulations.
    5. nmr analysis of the solution structure of the FHA domain of human Ki67 and mapping of the binding surface for NIFK binding
      Title: Structure of human Ki67 FHA domain and its binding to a phosphoprotein fragment from hNIFK reveal unique recognition sites and new views to the structural basis of FHA domain functions.
    Submit: New GeneRIF Correction

    Phenotypes

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    Find tests for this gene in the NIH Genetic Testing Registry (GTR)

    Review eQTL and phenotype association data in this region using PheGenI

    Variation

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    See variants in ClinVar

    See studies and variants in dbVar

    See Variation Viewer (GRCh37.p13)

    See Variation Viewer (GRCh38)

    Genotypes

    HIV-1 interactions

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    Protein interactions

    Protein Gene Interaction Pubs
    Pr55(Gag) gag Tandem affinity purification and mass spectrometry analysis identify Nopp34 associated HIV-1 Gag in 293T cells PubMed
    retropepsin gag-pol Positional proteomics analysis identifies the cleavage of human MKI67 (FHA domain) interacting nucleolar phosphoprotein (MKI67IP) at amino acid residues 188-189 by the HIV-1 protease PubMed

    Go to the HIV-1, Human Interaction Database

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    enables RNA binding HDA PubMed 
    enables RNA binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables RNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in protein-containing complex assembly NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in rRNA metabolic process NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in rRNA transcription NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in chromosome IDA
    Inferred from Direct Assay
    more info
    		  
    located_in condensed nuclear chromosome IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in nucleolus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in nucleolus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    MKI67 FHA domain-interacting nucleolar phosphoprotein
    Names
    hNIFK
    nucleolar phosphoprotein Nopp34
    nucleolar protein interacting with the FHA domain of pKi-67

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_032390.5 → NP_115766.3  MKI67 FHA domain-interacting nucleolar phosphoprotein

      See identical proteins and their annotated locations for NP_115766.3

      Status: REVIEWED

      Source sequence(s)
      BC022990, BC024238, BE671589
      Consensus CDS
      CCDS2135.1
      UniProtKB/Swiss-Prot
      A8K788, Q8TB66, Q96ED4, Q9BYG3
      Related
      ENSP00000285814.4, ENST00000285814.9
      Conserved Domains (3) summary
      cd12307
      Location:46 → 119
      RRM_NIFK_like; RNA recognition motif in nucleolar protein interacting with the FHA domain of pKI-67 (NIFK) and similar proteins
      pfam12196
      Location:227 → 266
      hNIFK_binding; FHA Ki67 binding domain of hNIFK
      cl25887
      Location:2 → 151
      RRM; RNA recognition motif (RRM) domain [Translation, ribosomal structure and biogenesis]

    RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2023_10

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh38.p14 Primary Assembly

    Genomic

    1. NC_000002.12 Reference GRCh38.p14 Primary Assembly

      Range
      121726945..121736875 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    Alternate T2T-CHM13v2.0

    Genomic

    1. NC_060926.1 Alternate T2T-CHM13v2.0

      Range
      122162220..122172150 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q9BYG3.1 GenPept UniProtKB/Swiss-Prot:Q9BYG3

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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