Actn2 actinin alpha 2 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Actn2provided by RGD
Official Full Name: actinin alpha 2provided by RGD
Primary source: RGD:1308097
See related: Ensembl:ENSRNOG00000017833 AllianceGenome:RGD:1308097
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Summary: Predicted to enable several functions, including cytoskeletal protein binding activity; nuclear receptor coactivator activity; and protein domain specific binding activity. Predicted to be involved in several processes, including focal adhesion assembly; muscle cell development; and regulation of ion transmembrane transporter activity. Predicted to act upstream of or within muscle contraction. Is active in glutamatergic synapse; postsynaptic density membrane; and postsynaptic density, intracellular component. Colocalizes with cortical actin cytoskeleton. Human ortholog(s) of this gene implicated in arrhythmogenic right ventricular cardiomyopathy and dilated cardiomyopathy 1AA. Orthologous to human ACTN2 (actinin alpha 2). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in Muscle (RPKM 4445.3) and Heart (RPKM 4260.4) See more
Orthologs: human mouse all
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Genomic context

Location:: 17q12.1

Exon count:: 21

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	17	NC_086035.1 (62835055..62902331)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	17	NC_051352.1 (58143334..58210622)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	17	NC_005116.4 (66304530..66397647, complement)

Chromosome 17 - NC_086035.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The actin binding protein alpha-actinin-2 expression is associated with dendritic spine plasticity and migrating granule cells in the rat dentate gyrus following pilocarpine-induced seizures.
Title: The actin binding protein α-actinin-2 expression is associated with dendritic spine plasticity and migrating granule cells in the rat dentate gyrus following pilocarpine-induced seizures.
Data suggest that kindlin-2 (Kind2/Fermt2) interacts with actin alpha 2 (Actn2) and integrin beta 1 (Itgb1) and co-localizes to cardiac sarcomere at Z-disc; knockdown of Kind2 leads to dissociation of Actn2 and Itgb1.
Title: Kindlin-2 interacts with α-actinin-2 and β1 integrin to maintain the integrity of the Z-disc in cardiac muscles.
ALpha-actinin-2, partially through its calcium insensitive EF-hand motif, nucleates post-synaptic density formation via F-actin organization and modulates spine maturation to mediate synaptogenesis.
Title: α-Actinin-2 mediates spine morphology and assembly of the post-synaptic density in hippocampal neurons.
Results support alpha-actinin adaptation at the isoform level and show that alpha-actinin-2 expression is associated with improvement of muscular aerobic capacity.
Title: Effects of ageing and endurance exercise training on alpha-actinin isoforms in rat plantaris muscle.
In contrast to alpha-actinin-3, exercise training increased alpha-actinin-2 expression levels in skeletal muscles, apparently related to the change to muscle with higher aerobic capacity.
Title: Different adaptations of alpha-actinin isoforms to exercise training in rat skeletal muscles.
CAMKII isoforms interact with the postsynaptic density proteins NR2B, densin-180, and alpha-actinin-2
Title: Multivalent interactions of calcium/calmodulin-dependent protein kinase II with the postsynaptic density proteins NR2B, densin-180, and alpha-actinin-2.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

d17rat149 (e-PCR)
- UniSTS:121216

Actn2 (e-PCR), detects polymorphism
- UniSTS:508559

ha2028 (e-PCR)
- UniSTS:515473

AW531528 (e-PCR)
- UniSTS:261828

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables FATZ binding	ISO Inferred from Sequence Orthology more info
enables LIM domain binding	ISO Inferred from Sequence Orthology more info
enables actin filament binding	IBA Inferred from Biological aspect of Ancestor more info
enables actin filament binding	ISO Inferred from Sequence Orthology more info
enables calcium ion binding	IEA Inferred from Electronic Annotation more info
enables cytoskeletal protein binding	ISO Inferred from Sequence Orthology more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables nuclear receptor coactivator activity	ISO Inferred from Sequence Orthology more info
enables phosphatidylinositol-4,5-bisphosphate binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein domain specific binding	ISO Inferred from Sequence Orthology more info
enables structural constituent of postsynaptic actin cytoskeleton	IDA Inferred from Direct Assay more info	PubMed
enables structural constituent of postsynaptic actin cytoskeleton	IMP Inferred from Mutant Phenotype more info	PubMed
enables titin Z domain binding	ISO Inferred from Sequence Orthology more info
enables titin binding	ISO Inferred from Sequence Orthology more info
enables transmembrane transporter binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in actin cytoskeleton organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in actin filament uncapping	ISO Inferred from Sequence Orthology more info
involved_in cardiac muscle cell development	ISO Inferred from Sequence Orthology more info
involved_in focal adhesion assembly	ISO Inferred from Sequence Orthology more info
involved_in microspike assembly	ISO Inferred from Sequence Orthology more info
involved_in muscle cell development	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of potassium ion transport	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of protein localization to cell surface	ISO Inferred from Sequence Orthology more info
involved_in phospholipase C-activating angiotensin-activated signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of DNA-templated transcription	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of endocytic recycling	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of potassium ion transport	ISO Inferred from Sequence Orthology more info
involved_in postsynaptic actin cytoskeleton organization	IEA Inferred from Electronic Annotation more info
involved_in protein localization to plasma membrane	ISO Inferred from Sequence Orthology more info
involved_in regulation of membrane potential	ISO Inferred from Sequence Orthology more info
involved_in sarcomere organization	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
is_active_in Z disc	IBA Inferred from Biological aspect of Ancestor more info
located_in Z disc	ISO Inferred from Sequence Orthology more info
is_active_in cell junction	IBA Inferred from Biological aspect of Ancestor more info
is_active_in cell projection	IBA Inferred from Biological aspect of Ancestor more info
located_in cortical actin cytoskeleton	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	TAS Traceable Author Statement more info
located_in filopodium	ISO Inferred from Sequence Orthology more info
located_in focal adhesion	ISO Inferred from Sequence Orthology more info
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in glutamatergic synapse	IMP Inferred from Mutant Phenotype more info	PubMed
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
is_active_in postsynaptic actin cytoskeleton	IDA Inferred from Direct Assay more info	PubMed
is_active_in postsynaptic density membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in postsynaptic density, intracellular component	IDA Inferred from Direct Assay more info	PubMed
located_in sarcomere	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: alpha-actinin-2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001170325.1 → NP_001163796.1 alpha-actinin-2

See identical proteins and their annotated locations for NP_001163796.1

Status: VALIDATED

Source sequence(s)

CK355337, CK358853, CK600096, CR461659, DY315226, FM118105, FM123616

UniProtKB/TrEMBL

A6KN31, D3ZCV0

Related

ENSRNOP00000024098.5, ENSRNOT00000024098.8

Conserved Domains (6) summary

PTZ00184
Location:747 → 851

PTZ00184; calmodulin; Provisional

cd00176
Location:402 → 629

SPEC; Spectrin repeats, found in several proteins involved in cytoskeletal structure; family members include spectrin, alpha-actinin and dystrophin; the spectrin repeat forms a three helix bundle with the second helix interrupted by proline in some sequences; ...

pfam00435
Location:281 → 391

Spectrin; Spectrin repeat

pfam08726
Location:824 → 890

EFhand_Ca_insen; Ca2+ insensitive EF hand

cd21214
Location:35 → 139

CH_ACTN_rpt1; first calponin homology (CH) domain found in the alpha-actinin family

cd21216
Location:143 → 257

CH_ACTN_rpt2; second calponin homology (CH) domain found in the alpha-actinin family