Gak cyclin G associated kinase [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Gakprovided by RGD
Official Full Name: cyclin G associated kinaseprovided by RGD
Primary source: RGD:621589
See related: Ensembl:ENSRNOG00000000048 AllianceGenome:RGD:621589
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Summary: Enables cyclin binding activity. Predicted to be involved in several processes, including Golgi to lysosome transport; clathrin coat disassembly; and clathrin-coated pit assembly. Predicted to act upstream of or within several processes, including Golgi organization; animal organ development; and positive regulation of neural precursor cell proliferation. Predicted to be located in Golgi apparatus. Predicted to be active in cytoplasm; intracellular membrane-bounded organelle; and vesicle. Orthologous to human GAK (cyclin G associated kinase). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in Kidney (RPKM 206.3), Thymus (RPKM 193.3) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 14p22

Exon count:: 28

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	14	NC_086032.1 (1234272..1308492)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	14	NC_051349.1 (1089853..1164098)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	14	NC_005113.4 (2100104..2174332)

Chromosome 14 - NC_086032.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Early stages of clathrin-mediated endocytosis (CME) were partially inhibited when GAK expression was knocked down; GAK plays a role in clathrin-dependent trafficking from the trans Golgi network.
Title: Multiple roles for cyclin G-associated kinase in clathrin-mediated sorting events.

Submit: New GeneRIF Correction

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

GAK__6772 (e-PCR)
- UniSTS:466196

RH131491 (e-PCR)
- UniSTS:214774

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables clathrin binding	IBA Inferred from Biological aspect of Ancestor more info
enables cyclin binding	IDA Inferred from Direct Assay more info	PubMed
enables protein kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in Golgi organization	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within Golgi organization	ISO Inferred from Sequence Orthology more info
involved_in Golgi organization	ISO Inferred from Sequence Orthology more info
involved_in Golgi to lysosome transport	IEA Inferred from Electronic Annotation more info
involved_in Golgi to lysosome transport	ISO Inferred from Sequence Orthology more info
involved_in clathrin coat assembly	IEA Inferred from Electronic Annotation more info
involved_in clathrin coat assembly	ISO Inferred from Sequence Orthology more info
involved_in clathrin coat disassembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in clathrin coat disassembly	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within clathrin coat disassembly	ISO Inferred from Sequence Orthology more info
involved_in clathrin coat disassembly	ISO Inferred from Sequence Orthology more info
involved_in clathrin-dependent endocytosis	IBA Inferred from Biological aspect of Ancestor more info
involved_in clathrin-dependent endocytosis	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within clathrin-dependent endocytosis	ISO Inferred from Sequence Orthology more info
involved_in clathrin-dependent endocytosis	ISO Inferred from Sequence Orthology more info
involved_in clathrin-dependent endocytosis	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within endoplasmic reticulum organization	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within epidermal cell differentiation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within epithelial cell development	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within establishment of skin barrier	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within forebrain morphogenesis	ISO Inferred from Sequence Orthology more info
involved_in intracellular transport	ISO Inferred from Sequence Orthology more info
involved_in intracellular transport	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within intrahepatic bile duct development	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within keratinocyte differentiation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of neuron projection development	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of neuron projection development	ISO Inferred from Sequence Orthology more info
acts_upstream_of neural precursor cell proliferation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within neuroepithelial cell differentiation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of neural precursor cell proliferation	ISO Inferred from Sequence Orthology more info
involved_in protein localization to Golgi apparatus	IEA Inferred from Electronic Annotation more info
involved_in protein localization to Golgi apparatus	ISO Inferred from Sequence Orthology more info
involved_in protein localization to plasma membrane	IEA Inferred from Electronic Annotation more info
involved_in protein localization to plasma membrane	ISO Inferred from Sequence Orthology more info
involved_in receptor-mediated endocytosis	ISO Inferred from Sequence Orthology more info
involved_in regulation of clathrin coat assembly	IEA Inferred from Electronic Annotation more info
involved_in regulation of clathrin coat assembly	ISO Inferred from Sequence Orthology more info
involved_in regulation of clathrin coat assembly	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within stem cell differentiation	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in Golgi apparatus	IEA Inferred from Electronic Annotation more info
located_in Golgi apparatus	ISO Inferred from Sequence Orthology more info
located_in clathrin-coated vesicle	IEA Inferred from Electronic Annotation more info
located_in clathrin-coated vesicle	ISO Inferred from Sequence Orthology more info
located_in clathrin-coated vesicle	ISS Inferred from Sequence or Structural Similarity more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in focal adhesion	IEA Inferred from Electronic Annotation more info
is_active_in intracellular membrane-bounded organelle	IBA Inferred from Biological aspect of Ancestor more info
located_in intracellular membrane-bounded organelle	ISO Inferred from Sequence Orthology more info
located_in perinuclear region of cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in vesicle	IBA Inferred from Biological aspect of Ancestor more info
located_in vesicle	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: cyclin-G-associated kinase

NP_112292.1

EC 2.7.11.1

XP_006250645.1

EC 2.7.11.1

XP_006250646.1

EC 2.7.11.1

XP_017454878.1

EC 2.7.11.1

XP_038948413.1

EC 2.7.11.1

XP_063129735.1

EC 2.7.11.1

XP_063129736.1

EC 2.7.11.1

XP_063129737.1

EC 2.7.11.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_031030.2 → NP_112292.1 cyclin-G-associated kinase

See identical proteins and their annotated locations for NP_112292.1

Status: PROVISIONAL

Source sequence(s)

D38560

UniProtKB/Swiss-Prot

P97874

UniProtKB/TrEMBL

F1LMD9

Related

ENSRNOP00000000064.1, ENSRNOT00000000064.3

Conserved Domains (4) summary

cd06257
Location:1253 → 1298

DnaJ; DnaJ domain or J-domain. DnaJ/Hsp40 (heat shock protein 40) proteins are highly conserved and play crucial roles in protein translation, folding, unfolding, translocation, and degradation. They act primarily by stimulating the ATPase activity of Hsp70s, ...

smart00220
Location:41 → 311

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd14036
Location:39 → 320

STKc_GAK; Catalytic domain of the Serine/Threonine protein kinase, cyclin G-Associated Kinase

pfam10409
Location:568 → 706

PTEN_C2; C2 domain of PTEN tumour-suppressor protein

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086032.1 Reference GRCr8

Range

1234272..1308492

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_063273665.1 → XP_063129735.1 cyclin-G-associated kinase isoform X1
XM_006250583.5 → XP_006250645.1 cyclin-G-associated kinase isoform X2

UniProtKB/TrEMBL

F1LMD9

Conserved Domains (4) summary

cd06257
Location:1226 → 1271

DnaJ; DnaJ domain or J-domain. DnaJ/Hsp40 (heat shock protein 40) proteins are highly conserved and play crucial roles in protein translation, folding, unfolding, translocation, and degradation. They act primarily by stimulating the ATPase activity of Hsp70s, ...

smart00220
Location:41 → 311

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd14036
Location:39 → 320

STKc_GAK; Catalytic domain of the Serine/Threonine protein kinase, cyclin G-Associated Kinase

pfam10409
Location:568 → 706

PTEN_C2; C2 domain of PTEN tumour-suppressor protein
XM_006250584.5 → XP_006250646.1 cyclin-G-associated kinase isoform X3

UniProtKB/TrEMBL

A0A8I5ZTC5, F1LMD9

Related

ENSRNOP00000081704.1

Conserved Domains (4) summary

cd06257
Location:1204 → 1249

DnaJ; DnaJ domain or J-domain. DnaJ/Hsp40 (heat shock protein 40) proteins are highly conserved and play crucial roles in protein translation, folding, unfolding, translocation, and degradation. They act primarily by stimulating the ATPase activity of Hsp70s, ...

smart00220
Location:41 → 311

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd14036
Location:39 → 320

STKc_GAK; Catalytic domain of the Serine/Threonine protein kinase, cyclin G-Associated Kinase

pfam10409
Location:568 → 706

PTEN_C2; C2 domain of PTEN tumour-suppressor protein
XM_063273666.1 → XP_063129736.1 cyclin-G-associated kinase isoform X4
XM_063273667.1 → XP_063129737.1 cyclin-G-associated kinase isoform X7
XM_017599389.3 → XP_017454878.1 cyclin-G-associated kinase isoform X5

UniProtKB/TrEMBL

A6KPE6, A6KPE7

Conserved Domains (4) summary

cd06257
Location:1092 → 1137

DnaJ; DnaJ domain or J-domain. DnaJ/Hsp40 (heat shock protein 40) proteins are highly conserved and play crucial roles in protein translation, folding, unfolding, translocation, and degradation. They act primarily by stimulating the ATPase activity of Hsp70s, ...

smart00220
Location:1 → 150

S_TKc; Serine/Threonine protein kinases, catalytic domain

pfam10409
Location:407 → 545

PTEN_C2; C2 domain of PTEN tumour-suppressor protein

cl21453
Location:1 → 159

PKc_like; Protein Kinases, catalytic domain
XM_039092485.2 → XP_038948413.1 cyclin-G-associated kinase isoform X6

Conserved Domains (3) summary

cd06257
Location:723 → 768

DnaJ; DnaJ domain or J-domain. DnaJ/Hsp40 (heat shock protein 40) proteins are highly conserved and play crucial roles in protein translation, folding, unfolding, translocation, and degradation. They act primarily by stimulating the ATPase activity of Hsp70s, ...

PHA03307
Location:289 → 608

PHA03307; transcriptional regulator ICP4; Provisional

pfam10409
Location:38 → 176

PTEN_C2; C2 domain of PTEN tumor-suppressor protein