BTF3 basic transcription factor 3 [Homo sapiens (human)] - Gene

Summary

Official Symbol: BTF3provided by HGNC
Official Full Name: basic transcription factor 3provided by HGNC
Primary source: HGNC:HGNC:1125
See related: Ensembl:ENSG00000145741 MIM:602542; AllianceGenome:HGNC:1125
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: NACB; BTF3a; BTF3b; BETA-NAC
Summary: This gene encodes the basic transcription factor 3. This protein forms a stable complex with RNA polymerase IIB and is required for transcriptional initiation. Alternative splicing results in multiple transcript variants encoding different isoforms. This gene has multiple pseudogenes. [provided by RefSeq, Jul 2008]
Expression: Ubiquitous expression in ovary (RPKM 354.7), bone marrow (RPKM 186.5) and 25 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: 5q13.2

Exon count:: 6

Annotation release	Status	Assembly	Chr	Location
RS_2023_10	current	GRCh38.p14 (GCF_000001405.40)	5	NC_000005.10 (73498442..73505667)
RS_2023_10	current	T2T-CHM13v2.0 (GCF_009914755.1)	5	NC_060929.1 (73979765..73986989)
105.20220307	previous assembly	GRCh37.p13 (GCF_000001405.25)	5	NC_000005.9 (72794267..72801492)

Chromosome 5 - NC_000005.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Ubiquitination-specific protease 7 enhances stemness of hepatocellular carcinoma by stabilizing basic transcription factor 3.
Title: Ubiquitination-specific protease 7 enhances stemness of hepatocellular carcinoma by stabilizing basic transcription factor 3.
BTF3 promotes proliferation and glycolysis in hepatocellular carcinoma by regulating GLUT1.
Title: BTF3 promotes proliferation and glycolysis in hepatocellular carcinoma by regulating GLUT1.
BTF3-mediated regulation of BMI1 promotes colorectal cancer through influencing epithelial-mesenchymal transition and stem cell-like traits.
Title: BTF3-mediated regulation of BMI1 promotes colorectal cancer through influencing epithelial-mesenchymal transition and stem cell-like traits.
BTF3 confers oncogenic activity in prostate cancer through transcriptional upregulation of Replication Factor C.
Title: BTF3 confers oncogenic activity in prostate cancer through transcriptional upregulation of Replication Factor C.
miR802 inhibits the epithelialmesenchymal transition, migration and invasion of cervical cancer by regulating BTF3.
Title: miR‑802 inhibits the epithelial‑mesenchymal transition, migration and invasion of cervical cancer by regulating BTF3.
BTF3 promotes stemness and inhibits TypeInterferon signaling pathway in triple-negative breast cancer.
Title: BTF3 promotes stemness and inhibits TypeⅠInterferon signaling pathway in triple-negative breast cancer.
BTF3 is positively related to CRC and BTF3-siRNA attenuated the tumorigenicity of colorectal cancer cells via MAD2L2, MCM3 and PLK1 activity reduction.
Title: Positive expression of basic transcription factor 3 predicts poor survival of colorectal cancer patients: possible mechanisms involved.
Expression of BTF3 is upregulated in hypopharyngeal squamous cell carcinoma and this upregulation is positively correlated with lymph node metastasis of this malignancy.
Title: Upregulation of BTF3 affects the proliferation, apoptosis, and cell cycle regulation in hypopharyngeal squamous cell carcinoma.
Study showed that BTF3 is overexpressed in prostate cancer (PCa) tissues and correlates with stem-like traits. Cancer stem-like characteristics in PCa including self-renewal and metastatic potential were impaired by BTF3 loss. BTF3 could stabilize BMI1, a crucial regulator of prostate stem cell self-renewal. More importantly, data revealed that BTF3 is highly predictive of poor prognosis.
Title: BTF3 sustains cancer stem-like phenotype of prostate cancer via stabilization of BMI1.
TCGA analysis reveals high expression levels of BTF3 in luminal/ER + breast cancer and cell line models harboring ERalpha overexpression. Concordantly, BTF3 expression is highly and strongly associated with ESR1 expression in multiple breast cancer cohorts.
Title: Inhibition of BTF3 sensitizes luminal breast cancer cells to PI3Kα inhibition through the transcriptional regulation of ERα.

Submit: New GeneRIF Correction See all GeneRIFs (18)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

HIV-1 interactions

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Replication interactions

Interaction	Pubs
Knockdown of basic transcription factor 3 (BTF3) by shRNA library screening inhibits HIV-1 replication in cultured Jurkat T-cells	PubMed

Protein interactions

Protein	Gene	Interaction	Pubs
integrase	gag-pol	Using acetylated HIV-1 IN as bait in yeast two-hybrid screening identifies transcription regulatory and chromatin remodeling factors LEDGF/p75, KAP1, BTF3b, THRAP3, and HMGN2 as IN-binding partners	PubMed

Go to the HIV-1, Human Interaction Database

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

STS-N23069 (e-PCR)
- UniSTS:16920

STS-N27423 (e-PCR)
- UniSTS:24121

G42974 (e-PCR)
- UniSTS:94851

MARC_7241-7242:996687679:1 (e-PCR)
- UniSTS:269746

MARC_7241-7242:1007583003:3 (e-PCR)
- UniSTS:269746

RH70088 (e-PCR)
- UniSTS:65779

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables RNA binding	HDA more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in in utero embryonic development	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of protein localization to endoplasmic reticulum	IDA Inferred from Direct Assay more info	PubMed
involved_in protein transport	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	IDA Inferred from Direct Assay more info
part_of nascent polypeptide-associated complex	IBA Inferred from Biological aspect of Ancestor more info
part_of nascent polypeptide-associated complex	IPI Inferred from Physical Interaction more info	PubMed
located_in nucleus	ISS Inferred from Sequence or Structural Similarity more info

General protein information

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Preferred Names: transcription factor BTF3

Names: NAC-beta; RNA polymerase B transcription factor 3; nascent polypeptide-associated complex subunit beta; nascent-polypeptide-associated complex beta polypeptide

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001037637.2 → NP_001032726.1 transcription factor BTF3 isoform A

See identical proteins and their annotated locations for NP_001032726.1

Status: REVIEWED

Description

Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (A).

Source sequence(s)

AC099522, BG182166, BU661989, BX359147

Consensus CDS

CCDS34185.1

UniProtKB/Swiss-Prot

A8K510, P20290, Q13893, Q76M56

Related

ENSP00000369965.3, ENST00000380591.8

Conserved Domains (1) summary

pfam01849
Location:87 → 140

NAC; NAC domain
NM_001207.5 → NP_001198.2 transcription factor BTF3 isoform B

See identical proteins and their annotated locations for NP_001198.2

Status: REVIEWED

Description

Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1, resulting in translation initiation at a downstream AUG and a protein (isoform B) with a shorter N-terminus, compared to isoform A.

Source sequence(s)

AC099522, BC008062, BG182166, BU661989

Consensus CDS

CCDS4019.1

UniProtKB/TrEMBL

A0A7I2V3T6

Related

ENSP00000338516.8, ENST00000335895.12

Conserved Domains (1) summary

cd22055
Location:9 → 125

NAC_BTF3; basal transcription factor BTF3
NM_001393652.1 → NP_001380581.1 transcription factor BTF3 isoform C

Status: REVIEWED

Source sequence(s)

AC099522
NM_001393653.1 → NP_001380582.1 transcription factor BTF3 isoform D

Status: REVIEWED

Source sequence(s)

AC099522

UniProtKB/TrEMBL

A0A7I2YQL2

Related

ENSP00000503967.1, ENST00000676862.1