E4f1 E4F transcription factor 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: E4f1provided by MGI
Official Full Name: E4F transcription factor 1provided by MGI
Primary source: MGI:MGI:109530
See related: Ensembl:ENSMUSG00000024137 AllianceGenome:MGI:109530
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: p120E4F; phi-AP3
Summary: This gene encodes a member of the GLI-Kruppel zinc finger family. The encoded protein is likely to be multi-functional, with both adenovirus E1A-regulated transcription factor and ubiquitin E3 ligase activities, including roles in cell cycle regulation and the ubiquitination of p53. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2014]
Expression: Ubiquitous expression in ovary adult (RPKM 25.6), thymus adult (RPKM 24.6) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 17 A3.3; 17 12.39 cM

Exon count:: 13

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	17	NC_000083.7 (24662752..24674366, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	17	NC_000083.6 (24443778..24455392, complement)

Chromosome 17 - NC_000083.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The multifunctional protein E4F1 links P53 to lipid metabolism in adipocytes.
Title: The multifunctional protein E4F1 links P53 to lipid metabolism in adipocytes.
these data identify E4F1 as a master regulator of the PDC.
Title: E4F1 controls a transcriptional program essential for pyruvate dehydrogenase activity.
These data reveal a central role for Dlat in the metabolic program regulated by E4F1 in basal keratinocytes.
Title: E4F1-mediated control of pyruvate dehydrogenase activity is essential for skin homeostasis.
Acute inactivation of E4F1 in these cells results in CHK1-dependent checkpoint deficiency and multiple mitochondrial dysfunctions that lead to increased ROS production, energy stress, and inhibition of de novo pyrimidine synthesis
Title: The transcription factor E4F1 coordinates CHK1-dependent checkpoint and mitochondrial functions.
Altogether, our results classify E4F1 as a master regulator of CHK1 activity that ensures high fidelity of DNA replication, thus safeguarding genome stability.
Title: E4F1 is a master regulator of CHK1-mediated functions.
deletion of E4F1 resulted in the death of histiocytic sarcoma cells and tumor regression in vivo and extended the lifespan
Title: E4F1 deficiency results in oxidative stress-mediated cell death of leukemic cells.
identify a regulatory axis essential for epidermal stem cell-dependent skin homeostasis implicating E4F1 and the Bmi1-Arf-p53 pathway.
Title: Transcription factor E4F1 is essential for epidermal stem cell maintenance and skin homeostasis.
Results establish a crucial role for E4F during early embryonic cell cycles and reveal an unexpected function for E4F in mitosis.
Title: The E4F protein is required for mitotic progression during embryonic cell cycles.

Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (4) 1 citation
Endonuclease-mediated (2)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

E4f1 (e-PCR), detects polymorphism
- UniSTS:506872

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables DNA-binding transcription activator activity, RNA polymerase II-specific	ISO Inferred from Sequence Orthology more info
enables DNA-binding transcription factor activity	IBA Inferred from Biological aspect of Ancestor more info
enables DNA-binding transcription repressor activity, RNA polymerase II-specific	ISO Inferred from Sequence Orthology more info
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables RNA polymerase II transcription regulatory region sequence-specific DNA binding	ISO Inferred from Sequence Orthology more info
enables RNA polymerase II-specific DNA-binding transcription factor binding	ISO Inferred from Sequence Orthology more info
enables cAMP response element binding	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within DNA replication	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within cell cycle	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within cell division	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within embryo development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of DNA-templated transcription	TAS Traceable Author Statement more info	PubMed
involved_in regulation of cell cycle process	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of cell cycle process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of mitotic cell cycle, embryonic	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in nuclear body	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in spindle	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: transcription factor E4F1

Names: RING-type E3 ubiquitin transferase E4F1; putative E3 ubiquitin-protein ligase E4F1; transcription factor E4F; transcription factor phi AP3

NP_001288713.1

EC 2.3.2.27

NP_001342637.1

EC 2.3.2.27

NP_031919.2

EC 2.3.2.27

XP_036016188.1

EC 2.3.2.27

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001301784.1 → NP_001288713.1 transcription factor E4F1 isoform 1

See identical proteins and their annotated locations for NP_001288713.1

Status: REVIEWED

Description

Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1).

Source sequence(s)

AC154237, AK033285, BC031757, BC057011, BY310538

UniProtKB/Swiss-Prot

Q05BH7, Q3UNJ9, Q62065, Q6IR08, Q6PGI1, Q8CCE9, Q9QY56

UniProtKB/TrEMBL

A0A2I3BR50

Related

ENSMUSP00000154438.2, ENSMUST00000228882.2

Conserved Domains (2) summary

COG5048
Location:193 → 594

COG5048; FOG: Zn-finger [General function prediction only]

sd00017
Location:520 → 540

ZF_C2H2; C2H2 Zn finger [structural motif]
NM_001355708.1 → NP_001342637.1 transcription factor E4F1 isoform 3

Status: REVIEWED

Source sequence(s)

AC154237, BC031757

UniProtKB/TrEMBL

A0A2I3BR50

Conserved Domains (2) summary

COG5048
Location:34 → 434

COG5048; FOG: Zn-finger [General function prediction only]

sd00017
Location:360 → 380

ZF_C2H2; C2H2 Zn finger [structural motif]
NM_007893.4 → NP_031919.2 transcription factor E4F1 isoform 2

See identical proteins and their annotated locations for NP_031919.2

Status: REVIEWED

Description

Transcript Variant: This variant (2) uses an alternate in-frame splice site in the central coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1.

Source sequence(s)

AC154237, AK033285, BC031757, BY310538

Consensus CDS

CCDS28481.1

UniProtKB/TrEMBL

A0A2I3BR50

Related

ENSMUSP00000062344.8, ENSMUST00000056032.9

Conserved Domains (4) summary

COG5048
Location:193 → 593

COG5048; FOG: Zn-finger [General function prediction only]

sd00017
Location:519 → 539

ZF_C2H2; C2H2 Zn finger [structural motif]

pfam00096
Location:221 → 243

zf-C2H2; Zinc finger, C2H2 type

pfam13465
Location:559 → 583

zf-H2C2_2; Zinc-finger double domain

RNA

NR_125990.1 RNA Sequence

Status: REVIEWED

Description

Transcript Variant: This variant (3) uses an alternate splice site in the central region compared to variant 1. This variant is represented as non-coding because the use of the 5'-most supported translational start codon, as used in variant 1, renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).

Source sequence(s)

AC154237, AK033285, AK077014, BC031757, BC057011, BY310538

Related

ENSMUST00000226754.2
NR_188837.1 RNA Sequence

Status: REVIEWED

Source sequence(s)

AC154237
NR_188838.1 RNA Sequence

Status: REVIEWED

Source sequence(s)

AC154237

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000083.7 Reference GRCm39 C57BL/6J

Range

24662752..24674366 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036160295.1 → XP_036016188.1 transcription factor E4F1 isoform X2

UniProtKB/TrEMBL

A0A2I3BPE3

Conserved Domains (3) summary

sd00017
Location:195 → 215

ZF_C2H2; C2H2 Zn finger [structural motif]

pfam00096
Location:221 → 243

zf-C2H2; Zinc finger, C2H2 type

pfam13465
Location:235 → 259

zf-H2C2_2; Zinc-finger double domain