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    PSH1 ubiquitin-protein ligase PSH1 [ Saccharomyces cerevisiae S288C ]

    Gene ID: 854102, updated on 17-May-2024

    Summary

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    Gene symbol
    PSH1
    Gene description
    ubiquitin-protein ligase PSH1
    Primary source
    FungiDB:YOL054W
    Locus tag
    YOL054W
    See related
    SGD:S000005415; AllianceGenome:SGD:S000005415
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Summary
    Enables ubiquitin-protein transferase activity. Involved in ubiquitin-dependent protein catabolic process. Located in chromosome, centromeric region. Orthologous to human TRIM25 (tripartite motif containing 25). [provided by Alliance of Genome Resources, Apr 2022]
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    Genomic context

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    Location:
    chromosome: XV
    Exon count:
    1
    Sequence:
    Chromosome: XV; NC_001147.6 (228614..229834)

    Chromosome XV - NC_001147.6Genomic Context describing neighboring genes Neighboring gene tRNA-Gly Neighboring gene tRNA-Asn Neighboring gene Aim39p Neighboring gene Ddr2p

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001147.6

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Molecular basis for the selective recognition and ubiquitination of centromeric histone H3 by yeast E3 ligase Psh1. Zhou N, et al. J Genet Genomics, 2021 Jun 20. PMID 34217622
    2. Quantitative proteomics and transcriptomics reveal key metabolic processes associated with cotton fiber initiation. Wang XC, et al. J Proteomics, 2015 Jan 30. PMID 25449837
    3. Ubiquitin protease Ubp8 is necessary for S. cerevisiae respiration. Leo M, et al. Biochim Biophys Acta Mol Cell Res, 2018 Aug 2. PMID 30077637
    4. N-terminal Sumoylation of Centromeric Histone H3 Variant Cse4 Regulates Its Proteolysis To Prevent Mislocalization to Non-centromeric Chromatin. Ohkuni K, et al. G3 (Bethesda), 2018 Mar 28. PMID 29432128, Free PMC Article
    5. SAGA DUB-Ubp8 Deubiquitylates Centromeric Histone Variant Cse4. Canzonetta C, et al. G3 (Bethesda), 2015 Nov 27. PMID 26613948, Free PMC Article

    See all (76) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Psh1p is required for the faithful inheritance of both centromeric and 2 mum plasmids.
      Title: The Ubiquitin Ligase (E3) Psh1p Is Required for Proper Segregation of both Centromeric and Two-Micron Plasmids in Saccharomyces cerevisiae.
    2. Psh1 and Ubr2 synergistically control the amount of proteins at the kinetochore.
      Title: Synergistic Control of Kinetochore Protein Levels by Psh1 and Ubr2.
    3. Report show that Fpr3 regulates Cse4 proteolysis and the structural changes in CSe4 might be important for its interaction with Psh1.
      Title: Degradation of centromeric histone H3 variant Cse4 requires the Fpr3 peptidyl-prolyl Cis-Trans isomerase.
    4. phosphorylation is crucial in Psh1-assisted control of Cse4 levels and the Psh1-Cse4 association itself functions to prevent Cse4 misincorporation
      Title: Phosphorylation by casein kinase 2 facilitates Psh1 protein-assisted degradation of Cse4 protein.
    5. The interaction between Psh1 and Spt16 is critical for both CENP-A(Cse4) ubiquitylation and its exclusion from euchromatin
      Title: The FACT complex interacts with the E3 ubiquitin ligase Psh1 to prevent ectopic localization of CENP-A.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ubiquitin protein ligase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables ubiquitin-protein transferase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables zinc ion binding RCA
    inferred from Reviewed Computational Analysis
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in proteasome-mediated ubiquitin-dependent protein catabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in ubiquitin-dependent protein catabolic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in ubiquitin-dependent protein catabolic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in chromosome, centromeric region IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleus HDA PubMed 
    located_in nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    ubiquitin-protein ligase PSH1
    NP_014587.1
    • E3 ubiquitin ligase targeting centromere-binding protein Cse4p; mediates polyubiquitination and degradation of histone H3 variant Cse4p; ubiquitination of Cse4p may be antagonized by Scm3p; interacts with the FACT complex preventing mislocalization of Cse4p to euchromatin independent of Slx5p; formyl-methionine/N-recognin component of N-end rule pathway mediating destruction of formylated cytosolic proteins elevated in response to starvation or stationary phase entry

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001147.6 Reference assembly

      Range
      228614..229834
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001183309.1 → NP_014587.1  TPA: ubiquitin-protein ligase PSH1 [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_014587.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6W213, Q12161
      UniProtKB/TrEMBL
      A0A8H4BUK4, A6ZNF6, B3LJ05, C7GK21, C8ZHQ9, N1NVP3
      Conserved Domains (2) summary
      cd16568
      Location:29 → 72
      RING-HC_ScPSH1_like; RING finger, HC subclass, found in Saccharomyces cerevisiae POB3/SPT16 histone-associated protein 1 (ScPSH1), Arabidopsis thaliana Protein KEEP ON GOING (AtKEG) and similar proteins
      cl00874
      Location:149 → 175
      DNA_RNApol_7kD; DNA directed RNA polymerase, 7 kDa subunit
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q12161.1

    Gene LinkOut

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