Rapgef2 Rap guanine nucleotide exchange factor 2 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Rapgef2provided by RGD
Official Full Name: Rap guanine nucleotide exchange factor 2provided by RGD
Primary source: RGD:1306434
See related: AllianceGenome:RGD:1306434
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: PDZ-GEF1; RA-GEF-1; nRap GEP
Summary: Enables protein kinase binding activity. Involved in several processes, including cell surface receptor signaling pathway; positive regulation of ERK1 and ERK2 cascade; and positive regulation of GTPase activity. Located in several cellular components, including neuron projection; neuronal cell body; and synapse. Part of protein-containing complex. Human ortholog(s) of this gene implicated in familial adult myoclonic epilepsy 7. Orthologous to human RAPGEF2 (Rap guanine nucleotide exchange factor 2). [provided by Alliance of Genome Resources, Apr 2022]
Annotation information: Annotation category: suggests misassembly
Expression: Biased expression in Heart (RPKM 122.9), Thymus (RPKM 110.5) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 2q33

Exon count:: 37

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	2	NC_086020.1 (166505868..166728139, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	2	NC_051337.1 (164207513..164322157, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	2	NC_005101.4 (177836191..178057378, complement)

Chromosome 2 - NC_086020.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Oncostatin M induces hyperalgesic priming and amplifies signaling of cAMP to ERK by RapGEF2 and PKA.
Title: Oncostatin M induces hyperalgesic priming and amplifies signaling of cAMP to ERK by RapGEF2 and PKA.
Following cAMP elevation, neuritogenic cyclic AMP sensor/Rapgef2 is activated for signaling to ERK to mediate neuritogenesis, whereas Epac2 is activated for signaling to the MAP kinase p38 to mediate growth arrest.
Title: Separate cyclic AMP sensors for neuritogenesis, growth arrest, and survival of neuroendocrine cells.
Data indicate that knockdown of Rapgef2 ablates cAMP-dependent ERK activation and neuritogenesis in neuronal cell line Neuroscreen-1 (NS-1).
Title: Rapgef2 connects GPCR-mediated cAMP signals to ERK activation in neuronal and endocrine cells.

Submit: New GeneRIF Correction

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables GTPase activator activity	ISO Inferred from Sequence Orthology more info
enables PDZ domain binding	ISO Inferred from Sequence Orthology more info
enables PDZ domain binding	ISS Inferred from Sequence or Structural Similarity more info
enables WW domain binding	ISO Inferred from Sequence Orthology more info
enables WW domain binding	ISS Inferred from Sequence or Structural Similarity more info
enables beta-1 adrenergic receptor binding	ISO Inferred from Sequence Orthology more info
enables beta-1 adrenergic receptor binding	ISS Inferred from Sequence or Structural Similarity more info
NOT enables cAMP binding	ISO Inferred from Sequence Orthology more info
enables cAMP binding	ISO Inferred from Sequence Orthology more info
enables cAMP binding	ISS Inferred from Sequence or Structural Similarity more info
NOT enables cGMP binding	ISO Inferred from Sequence Orthology more info
enables guanyl-nucleotide exchange factor activity	IBA Inferred from Biological aspect of Ancestor more info
enables guanyl-nucleotide exchange factor activity	ISO Inferred from Sequence Orthology more info
enables guanyl-nucleotide exchange factor activity	ISS Inferred from Sequence or Structural Similarity more info
enables phosphatidic acid binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in G protein-coupled receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in G protein-coupled receptor signaling pathway	ISS Inferred from Sequence or Structural Similarity more info
involved_in Rap protein signal transduction	ISO Inferred from Sequence Orthology more info
involved_in Rap protein signal transduction	ISS Inferred from Sequence or Structural Similarity more info
involved_in Ras protein signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in adenylate cyclase-activating adrenergic receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in adenylate cyclase-activating adrenergic receptor signaling pathway	ISS Inferred from Sequence or Structural Similarity more info
involved_in blood vessel development	ISO Inferred from Sequence Orthology more info
involved_in blood vessel development	ISS Inferred from Sequence or Structural Similarity more info
involved_in brain-derived neurotrophic factor receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cAMP-mediated signaling	ISO Inferred from Sequence Orthology more info
involved_in cAMP-mediated signaling	ISS Inferred from Sequence or Structural Similarity more info
involved_in cellular response to cAMP	ISO Inferred from Sequence Orthology more info
involved_in cellular response to cAMP	ISS Inferred from Sequence or Structural Similarity more info
involved_in cellular response to cGMP	ISO Inferred from Sequence Orthology more info
involved_in cellular response to cGMP	ISS Inferred from Sequence or Structural Similarity more info
involved_in cellular response to nerve growth factor stimulus	IDA Inferred from Direct Assay more info	PubMed
involved_in establishment of endothelial barrier	ISO Inferred from Sequence Orthology more info
involved_in establishment of endothelial barrier	ISS Inferred from Sequence or Structural Similarity more info
involved_in establishment of endothelial intestinal barrier	ISO Inferred from Sequence Orthology more info
involved_in forebrain neuron development	ISO Inferred from Sequence Orthology more info
involved_in forebrain neuron development	ISS Inferred from Sequence or Structural Similarity more info
involved_in microvillus assembly	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of cell population proliferation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of cell population proliferation	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of dendrite morphogenesis	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of dendrite morphogenesis	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of melanin biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of melanin biosynthetic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in nerve growth factor signaling pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in neuron migration	ISO Inferred from Sequence Orthology more info
involved_in neuron migration	ISS Inferred from Sequence or Structural Similarity more info
involved_in neuron projection development	ISO Inferred from Sequence Orthology more info
involved_in neuron projection development	ISS Inferred from Sequence or Structural Similarity more info
involved_in neuropeptide signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in neuropeptide signaling pathway	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of ERK1 and ERK2 cascade	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of ERK1 and ERK2 cascade	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of ERK1 and ERK2 cascade	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of dendritic cell apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of dendritic cell apoptotic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of neuron migration	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of neuron migration	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of neuron projection development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of vasculogenesis	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of vasculogenesis	ISS Inferred from Sequence or Structural Similarity more info
involved_in protein localization to plasma membrane	ISO Inferred from Sequence Orthology more info
involved_in regulation of cell junction assembly	ISO Inferred from Sequence Orthology more info
involved_in regulation of cell junction assembly	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of modification of postsynaptic structure	ISO Inferred from Sequence Orthology more info
involved_in regulation of synaptic plasticity	IDA Inferred from Direct Assay more info	PubMed
involved_in ventricular system development	ISO Inferred from Sequence Orthology more info
involved_in ventricular system development	ISS Inferred from Sequence or Structural Similarity more info

Component	Evidence Code	Pubs
is_active_in apical plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in apical plasma membrane	ISO Inferred from Sequence Orthology more info
located_in bicellular tight junction	ISO Inferred from Sequence Orthology more info
located_in cell-cell junction	ISO Inferred from Sequence Orthology more info
located_in cell-cell junction	ISS Inferred from Sequence or Structural Similarity more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	ISS Inferred from Sequence or Structural Similarity more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
is_active_in endocytic vesicle	IBA Inferred from Biological aspect of Ancestor more info
located_in endocytic vesicle	ISO Inferred from Sequence Orthology more info
located_in late endosome	ISO Inferred from Sequence Orthology more info
located_in late endosome	ISS Inferred from Sequence or Structural Similarity more info
located_in membrane	ISO Inferred from Sequence Orthology more info
located_in neuron projection	IDA Inferred from Direct Assay more info	PubMed
located_in neuronal cell body	IDA Inferred from Direct Assay more info	PubMed
located_in perinuclear region of cytoplasm	ISO Inferred from Sequence Orthology more info
located_in perinuclear region of cytoplasm	ISS Inferred from Sequence or Structural Similarity more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
is_active_in postsynapse	ISO Inferred from Sequence Orthology more info
part_of protein-containing complex	IDA Inferred from Direct Assay more info	PubMed
located_in synapse	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: rap guanine nucleotide exchange factor 2

Names: CNrasGEF; PDZ domain-containing guanine nucleotide exchange factor 1; Rap guanine nucleotide exchange factor (GEF) 2; cyclic nucleotide ras GEF; neural RAP guanine nucleotide exchange protein; rap guanine nucleotide exchange factor 6-like; ras/Rap1-associating GEF-1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001107684.1 → NP_001101154.1 rap guanine nucleotide exchange factor 2 isoform 2

See identical proteins and their annotated locations for NP_001101154.1

Status: VALIDATED

Source sequence(s)

CH473976

Conserved Domains (4) summary

smart00229
Location:73 → 186

RasGEFN; Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif

cd00155
Location:519 → 723

RasGEF; Guanine nucleotide exchange factor for Ras-like small GTPases. Small GTP-binding proteins of the Ras superfamily function as molecular switches in fundamental events such as signal transduction, cytoskeleton dynamics and intracellular trafficking. ...

cd00992
Location:190 → 270

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...

cl00155
Location:412 → 496

UBQ; Ubiquitin-like proteins
NM_001428994.1 → NP_001415923.1 rap guanine nucleotide exchange factor 2 isoform 1

Status: VALIDATED

Source sequence(s)

JAXUCZ010000002

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086020.1 Reference GRCr8

Range

166505868..166728139 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_063281807.1 → XP_063137877.1 rap guanine nucleotide exchange factor 2 isoform X2
XM_063281808.1 → XP_063137878.1 rap guanine nucleotide exchange factor 2 isoform X3
XM_063281806.1 → XP_063137876.1 rap guanine nucleotide exchange factor 2 isoform X1
XM_063281809.1 → XP_063137879.1 rap guanine nucleotide exchange factor 2 isoform X4
XM_063281812.1 → XP_063137882.1 rap guanine nucleotide exchange factor 2 isoform X6
XM_063281811.1 → XP_063137881.1 rap guanine nucleotide exchange factor 2 isoform X6
XM_063281813.1 → XP_063137883.1 rap guanine nucleotide exchange factor 2 isoform X7
XM_063281810.1 → XP_063137880.1 rap guanine nucleotide exchange factor 2 isoform X5
XM_063281815.1 → XP_063137885.1 rap guanine nucleotide exchange factor 2 isoform X9
XM_063281814.1 → XP_063137884.1 rap guanine nucleotide exchange factor 2 isoform X8

UniProtKB/Swiss-Prot

D3ZD17, F1M386