TADA3 transcriptional adaptor 3 [Homo sapiens (human)] - Gene

Summary

Official Symbol: TADA3provided by HGNC
Official Full Name: transcriptional adaptor 3provided by HGNC
Primary source: HGNC:HGNC:19422
See related: Ensembl:ENSG00000171148 MIM:602945; AllianceGenome:HGNC:19422
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: ADA3; NGG1; hADA3; STAF54; TADA3L
Summary: DNA-binding transcriptional activator proteins increase the rate of transcription by interacting with the transcriptional machinery bound to the basal promoter in conjunction with adaptor proteins, possibly by acetylation and destabilization of nucleosomes. The protein encoded by this gene is a transcriptional activator adaptor and a component of the histone acetyl transferase (HAT) coactivator complex which plays a crucial role in chromatin modulation and cell cycle progression. Along with the other components of the complex, this protein links transcriptional activators bound to specific promoters, to histone acetylation and the transcriptional machinery. The protein is also involved in the stabilization and activation of the p53 tumor suppressor protein that plays a role in the cellular response to DNA damage. Alternate splicing results in multiple transcript variants of this gene. [provided by RefSeq, May 2013]
Expression: Ubiquitous expression in testis (RPKM 21.9), ovary (RPKM 20.9) and 25 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: 3p25.3

Exon count:: 10

Annotation release	Status	Assembly	Chr	Location
RS_2023_10	current	GRCh38.p14 (GCF_000001405.40)	3	NC_000003.12 (9779967..9793011, complement)
RS_2023_10	current	T2T-CHM13v2.0 (GCF_009914755.1)	3	NC_060927.1 (9771920..9784965, complement)
105.20220307	previous assembly	GRCh37.p13 (GCF_000001405.25)	3	NC_000003.11 (9821651..9834695, complement)

Chromosome 3 - NC_000003.12 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Transcriptional adaptor 3 influences the proliferative and invasive phenotypes of non-small cell lung cancer cells via regulating EMT.
Title: Transcriptional adaptor 3 influences the proliferative and invasive phenotypes of non-small cell lung cancer cells via regulating EMT.
ADA3 expression level elevates in non-small cell lung cancer and correlates with poor overall survival in non-small cell lung cancer patients
Title: Overexpression of Alteration/Deficiency in Activation 3 correlates with poor prognosis in non-small cell lung cancer.
a pro-apoptotic mechanism centered on the intrinsic (mitochondrial) pathway and regulated by PCAF/ADA3 can influence the progression from premalignant to malignant change, and thus act as a tumor suppression mechanism in gastric cancer.
Title: Down-regulation of a pro-apoptotic pathway regulated by PCAF/ADA3 in early stage gastric cancer.
These results reveal a novel RTK-AKT-p300-ADA3 signaling pathway involved in growth factor-induced cell cycle progression.
Title: Epidermal Growth Factor Receptor activation promotes ADA3 acetylation through the AKT-p300 pathway.
PCAF and ADA3 transcriptionally regulate PACS1 and PACS1 is a key regulator of BAX/BAK oligomerization and the intrinsic (mitochondrial) pathway to apoptosis.
Title: Epigenetic control of mitochondrial cell death through PACS1-mediated regulation of BAX/BAK oligomerization.
ADA3 overexpression enhances cell proliferation that is associated with increased expression of c-MYC. Expression patterns with respect to ADA3/c-MYC can divide patients into four significantly different subgroups, with c-MYC High and ADA3 Low status independently predicting poor survival in patients.
Title: ADA3 regulates normal and tumor mammary epithelial cell proliferation through c-MYC.
review on hADA3 with a comprehensive outlook on the molecular and functional roles of hADA3
Title: Tale of a multifaceted co-activator, hADA3: from embryogenesis to cancer and beyond.
Findings demonstrate that acetylation of ADA3 by its associated histone acetyltransferases is essential for its key role in histone acetylation and cell cycle progression.
Title: Acetylation of Mammalian ADA3 Is Required for Its Functional Roles in Histone Acetylation and Cell Proliferation.
results thus demonstrate that the catalytic activity of GCN5 is stimulated by subunits of the ADA2a- or ADA2b-containing HAT modules and is further increased by incorporation of the distinct HAT modules in the ATAC or SAGA holo-complexes
Title: Subunits of ADA-two-A-containing (ATAC) or Spt-Ada-Gcn5-acetyltrasferase (SAGA) Coactivator Complexes Enhance the Acetyltransferase Activity of GCN5.
PCAF and ADA3 regulate Bid processing via PACS2, to modulate the mitochondrial cell death pathway in response to hGrzB.
Title: A functional genomics screen identifies PCAF and ADA3 as regulators of human granzyme B-mediated apoptosis and Bid cleavage.

Submit: New GeneRIF Correction See all GeneRIFs (26)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D8S2279 (e-PCR)
- UniSTS:473907

WI-14334 (e-PCR)
- UniSTS:39652

PMC117179P1 (e-PCR)
- UniSTS:270317

RH78176 (e-PCR)
- UniSTS:49135

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables nuclear receptor binding	IPI Inferred from Physical Interaction more info	PubMed
enables nuclear receptor coactivator activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein domain specific binding	IPI Inferred from Physical Interaction more info	PubMed
enables transcription coactivator activity	IBA Inferred from Biological aspect of Ancestor more info
enables transcription coactivator activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in chromatin organization	IEA Inferred from Electronic Annotation more info
involved_in intracellular estrogen receptor signaling pathway	TAS Traceable Author Statement more info	PubMed
involved_in mitotic cell cycle	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of DNA-templated transcription	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of DNA-templated transcription	NAS Non-traceable Author Statement more info	PubMed
involved_in positive regulation of gene expression	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of DNA repair	NAS Non-traceable Author Statement more info	PubMed
involved_in regulation of DNA-templated transcription	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of DNA-templated transcription	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of DNA-templated transcription	NAS Non-traceable Author Statement more info	PubMed
involved_in regulation of RNA splicing	NAS Non-traceable Author Statement more info	PubMed
involved_in regulation of cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of cell cycle	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of cell division	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of cell division	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of embryonic development	ISO Inferred from Sequence Orthology more info
involved_in regulation of embryonic development	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of protein phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in regulation of protein stability	IEA Inferred from Electronic Annotation more info
involved_in regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of transcription by RNA polymerase II	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of tubulin deacetylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of tubulin deacetylation	ISS Inferred from Sequence or Structural Similarity more info

Component	Evidence Code	Pubs
part_of ATAC complex	IDA Inferred from Direct Assay more info	PubMed
part_of ATAC complex	ISO Inferred from Sequence Orthology more info
part_of SAGA complex	IBA Inferred from Biological aspect of Ancestor more info
part_of SAGA complex	IDA Inferred from Direct Assay more info	PubMed
part_of SAGA complex	NAS Non-traceable Author Statement more info	PubMed
located_in mitotic spindle	NAS Non-traceable Author Statement more info	PubMed
located_in nucleoplasm	IDA Inferred from Direct Assay more info
located_in nucleoplasm	TAS Traceable Author Statement more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
part_of transcription factor TFTC complex	IDA Inferred from Direct Assay more info	PubMed
part_of transcription factor TFTC complex	NAS Non-traceable Author Statement more info	PubMed

General protein information

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Preferred Names: transcriptional adapter 3

Names: ADA3 homolog; ADA3-like protein; alteration/deficiency in activation 3; epididymis secretory sperm binding protein

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001278270.2 → NP_001265199.1 transcriptional adapter 3 isoform a

See identical proteins and their annotated locations for NP_001265199.1

Status: REVIEWED

Description

Transcript Variant: This variant (3) uses an alternate splice site in the 5' UTR compared to variant 1. Variants 1 and 3 encode the same isoform (a).

Source sequence(s)

AK292264, BC009240, BC013433

Consensus CDS

CCDS2583.1

UniProtKB/Swiss-Prot

O75528, Q6FI83, Q9UFS2

UniProtKB/TrEMBL

A8K899

Related

ENSP00000393471.1, ENST00000440161.5

Conserved Domains (1) summary

pfam10198
Location:309 → 422

Ada3; Histone acetyltransferases subunit 3
NM_006354.5 → NP_006345.1 transcriptional adapter 3 isoform a

See identical proteins and their annotated locations for NP_006345.1

Status: REVIEWED

Description

Transcript Variant: This variant (1) encodes the longer protein. Variants 1 and 3 encode the same isoform (a).

Source sequence(s)

AC022382

Consensus CDS

CCDS2583.1

UniProtKB/Swiss-Prot

O75528, Q6FI83, Q9UFS2

UniProtKB/TrEMBL

A8K899

Related

ENSP00000307684.2, ENST00000301964.7

Conserved Domains (1) summary

pfam10198
Location:309 → 422

Ada3; Histone acetyltransferases subunit 3
NM_133480.4 → NP_597814.1 transcriptional adapter 3 isoform b

See identical proteins and their annotated locations for NP_597814.1

Status: REVIEWED

Description

Transcript Variant: This variant (2) lacks an exon and its 3' terminal exon extends past a splice site that is used in variant 1, that results in an early stop codon and a different 3' UTR, compared to variant 1. It encodes isoform b which is shorter at the C-terminus, compared to isoform a.

Source sequence(s)

AC022382

Consensus CDS

CCDS2584.1

UniProtKB/TrEMBL

A0A024R2D7, A8K899

Related

ENSP00000343649.2, ENST00000343450.2

Conserved Domains (1) summary

pfam10198
Location:309 → 368

Ada3; Histone acetyltransferases subunit 3

RNA

NR_103488.3 RNA Sequence

Status: REVIEWED

Description

Transcript Variant: This variant (4) lacks two alternate internal exons and uses alternate splice sites in the 5' terminal exon and in an internal exon, compared to variant 1. This variant is represented as non-coding because it lacks a large portion of the coding region, including the translational start codon, found in variant 1.

Source sequence(s)

AC022382

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2023_10

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

NC_000003.12 Reference GRCh38.p14 Primary Assembly

Range

9779967..9793011 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate T2T-CHM13v2.0

Genomic

NC_060927.1 Alternate T2T-CHM13v2.0

Range

9771920..9784965 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Suppressed Reference Sequence(s)

The following Reference Sequences have been suppressed. Explain

NM_133481.1: Suppressed sequence

Description

NM_133481.1: This RefSeq was permanently suppressed because it is a nonsense-mediated mRNA decay (NMD) candidate.