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    Zfp516 zinc finger protein 516 [ Mus musculus (house mouse) ]

    Gene ID: 329003, updated on 5-Mar-2024

    Summary

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    Official Symbol
    Zfp516provided by MGI
    Official Full Name
    zinc finger protein 516provided by MGI
    Primary source
    MGI:MGI:2443957
    See related
    Ensembl:ENSMUSG00000058881 AllianceGenome:MGI:2443957
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    Zfp26l; Znf516; mKIAA0222; D230016L03; C330029B10Rik
    Summary
    Enables DNA-binding transcription factor binding activity and cis-regulatory region sequence-specific DNA binding activity. Involved in several processes, including adipose tissue development; brown fat cell differentiation; and positive regulation of cold-induced thermogenesis. Located in nucleus. Is expressed in several structures, including alimentary system; brain; genitourinary system; respiratory system; and sensory organ. Orthologous to human ZNF516 (zinc finger protein 516). [provided by Alliance of Genome Resources, Apr 2022]
    Expression
    Ubiquitous expression in ovary adult (RPKM 8.7), limb E14.5 (RPKM 5.0) and 27 other tissues See more
    Orthologs
    human all
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    Genomic context

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    Location:
    18 E3; 18 57.39 cM
    Exon count:
    13
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 18 NC_000084.7 (82925324..83023439)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 18 NC_000084.6 (82909688..83005314)

    Chromosome 18 - NC_000084.7Genomic Context describing neighboring genes Neighboring gene RIKEN cDNA 4930445N18 gene Neighboring gene STARR-positive B cell enhancer ABC_E2515 Neighboring gene STARR-positive B cell enhancer ABC_E11008 Neighboring gene STARR-positive B cell enhancer ABC_E4256 Neighboring gene RIKEN cDNA 4930592I03 gene Neighboring gene predicted gene, 30889 Neighboring gene STARR-seq mESC enhancer starr_45235 Neighboring gene STARR-positive B cell enhancer ABC_E7620 Neighboring gene predicted gene, 41795 Neighboring gene predicted gene 10523 Neighboring gene RIKEN cDNA 1700095A13 gene

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. Zinc finger proteins orchestrate active gene silencing during embryonic stem cell differentiation. Kwak S, et al. Nucleic Acids Res, 2018 Jul 27. PMID 29846698, Free PMC Article
    2. LSD1 Interacts with Zfp516 to Promote UCP1 Transcription and Brown Fat Program. Sambeat A, et al. Cell Rep, 2016 Jun 14. PMID 27264172, Free PMC Article
    3. Cold-inducible Zfp516 activates UCP1 transcription to promote browning of white fat and development of brown fat. Dempersmier J, et al. Mol Cell, 2015 Jan 22. PMID 25578880, Free PMC Article
    4. PWWP2B Fine-Tunes Adipose Thermogenesis by Stabilizing HDACs in a NuRD Subcomplex. Yan L, et al. Adv Sci (Weinh), 2021 Aug. PMID 34180153, Free PMC Article
    5. Transcriptional Networks Controlled by NKX2-1 in the Development of Forebrain GABAergic Neurons. Sandberg M, et al. Neuron, 2016 Sep 21. PMID 27657450, Free PMC Article

    See all (32) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Zfp217 and Zfp516 facilitate Ctbp2-mediated repression on active embryonic stem cell genes during differentiation.
      Title: Zinc finger proteins orchestrate active gene silencing during embryonic stem cell differentiation.
    2. through interaction with Zfp516, LSD1 is recruited to UCP1 and other brown adipose tissue-enriched genes.
      Title: LSD1 Interacts with Zfp516 to Promote UCP1 Transcription and Brown Fat Program.
    3. Zfp516 directly binds to the proximal region of the UCP1 promoter, not to the enhancer region where other transcription factors bind, and interacts with PRDM16 to activate the UCP1 promoter.
      Title: Cold-inducible Zfp516 activates UCP1 transcription to promote browning of white fat and development of brown fat.
    Submit: New GeneRIF Correction

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Gene trapped (1) 

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA-binding transcription factor activity, RNA polymerase II-specific IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables DNA-binding transcription factor binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables RNA polymerase II cis-regulatory region sequence-specific DNA binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables cis-regulatory region sequence-specific DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in adipose tissue development IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in brown fat cell differentiation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in positive regulation of DNA-templated transcription IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in positive regulation of cold-induced thermogenesis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in regulation of DNA-templated transcription IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in response to cold IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    is_active_in nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    zinc finger protein 516

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001177464.1 → NP_001170935.1  zinc finger protein 516

      See identical proteins and their annotated locations for NP_001170935.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein.
      Source sequence(s)
      AC142100, BC053104, CJ101707
      Consensus CDS
      CCDS29380.1
      UniProtKB/Swiss-Prot
      Q7TSH3
      Related
      ENSMUSP00000071216.7, ENSMUST00000071233.7
      Conserved Domains (5) summary
      PHA03247
      Location:811 → 1036
      PHA03247; large tegument protein UL36; Provisional
      sd00017
      Location:36 → 56
      ZF_C2H2; C2H2 Zn finger [structural motif]
      pfam00096
      Location:34 → 56
      zf-C2H2; Zinc finger, C2H2 type
      pfam13465
      Location:48 → 73
      zf-H2C2_2; Zinc-finger double domain
      pfam13909
      Location:62 → 86
      zf-H2C2_5; C2H2-type zinc-finger domain

    2. NM_183033.2 → NP_898854.1  zinc finger protein 516

      See identical proteins and their annotated locations for NP_898854.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein.
      Source sequence(s)
      AC142100, BC053104, CJ101868
      Consensus CDS
      CCDS29380.1
      UniProtKB/Swiss-Prot
      Q7TSH3
      Related
      ENSMUSP00000126629.2, ENSMUST00000171238.8
      Conserved Domains (5) summary
      PHA03247
      Location:811 → 1036
      PHA03247; large tegument protein UL36; Provisional
      sd00017
      Location:36 → 56
      ZF_C2H2; C2H2 Zn finger [structural motif]
      pfam00096
      Location:34 → 56
      zf-C2H2; Zinc finger, C2H2 type
      pfam13465
      Location:48 → 73
      zf-H2C2_2; Zinc-finger double domain
      pfam13909
      Location:62 → 86
      zf-H2C2_5; C2H2-type zinc-finger domain

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000084.7 Reference GRCm39 C57BL/6J

      Range
      82925324..83023439
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_006526504.5 → XP_006526567.1  zinc finger protein 516 isoform X2

      Conserved Domains (4) summary
      PHA03247
      Location:811 → 1036
      PHA03247; large tegument protein UL36; Provisional
      sd00017
      Location:36 → 56
      ZF_C2H2; C2H2 Zn finger [structural motif]
      pfam00096
      Location:34 → 56
      zf-C2H2; Zinc finger, C2H2 type
      pfam13465
      Location:48 → 73
      zf-H2C2_2; Zinc-finger double domain

    2. XM_036161187.1 → XP_036017080.1  zinc finger protein 516 isoform X3

      Conserved Domains (3) summary
      sd00017
      Location:36 → 56
      ZF_C2H2; C2H2 Zn finger [structural motif]
      pfam00096
      Location:34 → 56
      zf-C2H2; Zinc finger, C2H2 type
      pfam13465
      Location:48 → 73
      zf-H2C2_2; Zinc-finger double domain

    3. XM_036161188.1 → XP_036017081.1  zinc finger protein 516 isoform X3

      Conserved Domains (3) summary
      sd00017
      Location:36 → 56
      ZF_C2H2; C2H2 Zn finger [structural motif]
      pfam00096
      Location:34 → 56
      zf-C2H2; Zinc finger, C2H2 type
      pfam13465
      Location:48 → 73
      zf-H2C2_2; Zinc-finger double domain

    4. XM_030250516.2 → XP_030106376.1  zinc finger protein 516 isoform X2

      Conserved Domains (4) summary
      PHA03247
      Location:811 → 1036
      PHA03247; large tegument protein UL36; Provisional
      sd00017
      Location:36 → 56
      ZF_C2H2; C2H2 Zn finger [structural motif]
      pfam00096
      Location:34 → 56
      zf-C2H2; Zinc finger, C2H2 type
      pfam13465
      Location:48 → 73
      zf-H2C2_2; Zinc-finger double domain

    5. XM_006526502.4 → XP_006526565.1  zinc finger protein 516 isoform X1

      See identical proteins and their annotated locations for XP_006526565.1

      UniProtKB/Swiss-Prot
      Q7TSH3
      Conserved Domains (5) summary
      PHA03247
      Location:811 → 1036
      PHA03247; large tegument protein UL36; Provisional
      sd00017
      Location:36 → 56
      ZF_C2H2; C2H2 Zn finger [structural motif]
      pfam00096
      Location:34 → 56
      zf-C2H2; Zinc finger, C2H2 type
      pfam13465
      Location:48 → 73
      zf-H2C2_2; Zinc-finger double domain
      pfam13909
      Location:62 → 86
      zf-H2C2_5; C2H2-type zinc-finger domain

    6. XM_006526500.4 → XP_006526563.1  zinc finger protein 516 isoform X1

      See identical proteins and their annotated locations for XP_006526563.1

      UniProtKB/Swiss-Prot
      Q7TSH3
      Conserved Domains (5) summary
      PHA03247
      Location:811 → 1036
      PHA03247; large tegument protein UL36; Provisional
      sd00017
      Location:36 → 56
      ZF_C2H2; C2H2 Zn finger [structural motif]
      pfam00096
      Location:34 → 56
      zf-C2H2; Zinc finger, C2H2 type
      pfam13465
      Location:48 → 73
      zf-H2C2_2; Zinc-finger double domain
      pfam13909
      Location:62 → 86
      zf-H2C2_5; C2H2-type zinc-finger domain

    7. XM_036161186.1 → XP_036017079.1  zinc finger protein 516 isoform X2

      Conserved Domains (4) summary
      PHA03247
      Location:811 → 1036
      PHA03247; large tegument protein UL36; Provisional
      sd00017
      Location:36 → 56
      ZF_C2H2; C2H2 Zn finger [structural motif]
      pfam00096
      Location:34 → 56
      zf-C2H2; Zinc finger, C2H2 type
      pfam13465
      Location:48 → 73
      zf-H2C2_2; Zinc-finger double domain

    8. XM_006526503.4 → XP_006526566.1  zinc finger protein 516 isoform X1

      See identical proteins and their annotated locations for XP_006526566.1

      UniProtKB/Swiss-Prot
      Q7TSH3
      Conserved Domains (5) summary
      PHA03247
      Location:811 → 1036
      PHA03247; large tegument protein UL36; Provisional
      sd00017
      Location:36 → 56
      ZF_C2H2; C2H2 Zn finger [structural motif]
      pfam00096
      Location:34 → 56
      zf-C2H2; Zinc finger, C2H2 type
      pfam13465
      Location:48 → 73
      zf-H2C2_2; Zinc-finger double domain
      pfam13909
      Location:62 → 86
      zf-H2C2_5; C2H2-type zinc-finger domain

    9. XM_006526501.5 → XP_006526564.1  zinc finger protein 516 isoform X1

      See identical proteins and their annotated locations for XP_006526564.1

      UniProtKB/Swiss-Prot
      Q7TSH3
      Conserved Domains (5) summary
      PHA03247
      Location:811 → 1036
      PHA03247; large tegument protein UL36; Provisional
      sd00017
      Location:36 → 56
      ZF_C2H2; C2H2 Zn finger [structural motif]
      pfam00096
      Location:34 → 56
      zf-C2H2; Zinc finger, C2H2 type
      pfam13465
      Location:48 → 73
      zf-H2C2_2; Zinc-finger double domain
      pfam13909
      Location:62 → 86
      zf-H2C2_5; C2H2-type zinc-finger domain

    10. XM_006526498.3 → XP_006526561.1  zinc finger protein 516 isoform X1

      See identical proteins and their annotated locations for XP_006526561.1

      UniProtKB/Swiss-Prot
      Q7TSH3
      Conserved Domains (5) summary
      PHA03247
      Location:811 → 1036
      PHA03247; large tegument protein UL36; Provisional
      sd00017
      Location:36 → 56
      ZF_C2H2; C2H2 Zn finger [structural motif]
      pfam00096
      Location:34 → 56
      zf-C2H2; Zinc finger, C2H2 type
      pfam13465
      Location:48 → 73
      zf-H2C2_2; Zinc-finger double domain
      pfam13909
      Location:62 → 86
      zf-H2C2_5; C2H2-type zinc-finger domain

    11. XM_006526499.5 → XP_006526562.1  zinc finger protein 516 isoform X1

      See identical proteins and their annotated locations for XP_006526562.1

      UniProtKB/Swiss-Prot
      Q7TSH3
      Conserved Domains (5) summary
      PHA03247
      Location:811 → 1036
      PHA03247; large tegument protein UL36; Provisional
      sd00017
      Location:36 → 56
      ZF_C2H2; C2H2 Zn finger [structural motif]
      pfam00096
      Location:34 → 56
      zf-C2H2; Zinc finger, C2H2 type
      pfam13465
      Location:48 → 73
      zf-H2C2_2; Zinc-finger double domain
      pfam13909
      Location:62 → 86
      zf-H2C2_5; C2H2-type zinc-finger domain
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q7TSH3.1 GenPept UniProtKB/Swiss-Prot:Q7TSH3

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