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Platform GPL8768 Query DataSets for GPL8768
Status Public on Jun 26, 2009
Title Univ Colorado Denver Mycobacterium tuberculosis 4.6K Operon Microarray
Technology type spotted oligonucleotide
Distribution non-commercial
Organisms Mycobacterium tuberculosis CDC1551; Mycobacterium tuberculosis H37Rv
Manufacturer Voskuil laboratory, University of Colorado Denver
Manufacture protocol 1.Oligos (purchased from Operon) are arrayed in Whatman 384-well V-bottom plates. Each well contains 600 pmol of 70-mer oligo.
2. Oligos are suspended in 3xSSC to 20 uM and centrifuged at 500 rpm for 2 minutes.
3. Plates are stored at 4oC overnight.
4. Incubate at RT for 30 minutes and shake for 5 min to aid resuspension of DNA.
5. Centrifuge plates at 500 rpm for 2 minutes.
6. Remove seals.
7. Print.
8. After completion of print, allow slides to dry through passive evaporation in a protected environment.
Support glass
Coating polysine
 
 
Contributor(s) Voskuil M
Submission date Jun 25, 2009
Last update date Jun 26, 2009
Contact name Ryan William Honaker
Organization name University of Colorado Denver
Department Microbiology
Lab Marty Voskuil
Street address 12800 E. 19th Ave.
City Aurora
State/province CO
ZIP/Postal code 80045
Country USA
 
Samples (36) GSM422038, GSM422039, GSM422040, GSM422041, GSM422042, GSM422043 
Series (1)
GSE16811 The unique roles of DosT and DosS in DosR regulon induction and Mycobacterium tuberculosis dormancy

Data table header descriptions
ID
Gene_Symbol Gene symbol
ORF Locus tag of strain H37Rv
ORF Locus tag of strain CDC-1551
Gene_Description Short description of gene
Oligo_ID Unique oligo identifier
SPOT_ID

Data table
ID Gene_Symbol ORF ORF Gene_Description Oligo_ID SPOT_ID
1 dnaA Rv0001 MT0001 chromosomal replication initiator protein T000001_01
2 dnaN Rv0002 MT0002 DNA polymerase III, [beta] subunit T000002_01
3 recF Rv0003 MT0003 DNA replication and SOS induction T000003_01
4 Rv0004 Rv0004 MT0004 conserved hypothetical protein T000004_01
5 gyrB Rv0005 MT0005 DNA gyrase subunit B T000005_01
6 gyrA Rv0006 MT0006 DNA gyrase subunit A T000006_01
7 Rv0007 Rv0007 conserved hypothetical protein T000007_01
8 Rv0008c Rv0008c MT0010 hypothetical protein T000008_01
9 ppiA Rv0009 MT0011 peptidyl-prolyl cis-trans isomerase T000009_01
10 Rv0010c Rv0010c MT0013 hypothetical protein T000010_01
11 Rv0011c Rv0011c MT0014 hypothetical protein T000011_01
12 Rv0012 Rv0012 MT0015 possible cell division protein T000012_01
13 pabA Rv0013 MT0016 p-aminobenzoate synthase glutamine amidotransferase T000013_01
14 pknB Rv0014c MT0017 serine-threonine protein kinase T000014_01
15 pknA Rv0015c MT0018 serine-threonine protein kinase T000015_01
16 pbpA Rv0016c MT0019 penicillin-binding protein T000016_01
17 rodA Rv0017c MT0020 FtsW/RodA/SpovE family T000017_01
18 ppp Rv0018c MT0021 putative phosphoprotein phosphatase T000018_01
19 Rv0019c Rv0019c MT0022 conserved hypothetical protein T000019_01
20 Rv0020c Rv0020c MT0023 conserved hypothetical protein T000020_01

Total number of rows: 4589

Table truncated, full table size 285 Kbytes.




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Supplementary data files not provided
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