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| Status |
Public on Jun 01, 2020 |
| Title |
Time-series transcription microarray data of Streptomyces coelicolor M145-OA |
| Organism |
Streptomyces coelicolor |
| Experiment type |
Expression profiling by array
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| Summary |
The present work aimed at selecting appropriate native temporal promoters with the identical profile of the cumate-inducible promoter under the optimal induction condition. This strategy enabled us to optimized the expression of biosynthetic gene cluster of desired secondary metabolites in Streptomyces, while avoid the use of inducer during fermentation. By clustering the genes with quite similar transcriptional profile with that of gfp controlled by the cumate-inducible promoter based on time-series microarray data, 50 qualified genes were identified, which were controlled by 24 putative promoters. Then, the cumate-inducible promoter were replaced by the 24 native temporal promoters, and fermentation results showed the good performance of these promoters compared to that of the cumate-inducible promoter. Therefore, our strategy could be used to fine-tune the expression of target BGCs for production improvement.
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| Overall design |
Transcriptional profiling of S. coelicolor M145-OA at different growth phases (exponential, transitional and stationary phase)
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| Contributor(s) |
Li S, Wang J, Wang W |
| Citation missing |
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| |
| Submission date |
Jun 22, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Keqian Yang |
| E-mail(s) |
yangkq@im.ac.cn
|
| Organization name |
IMCAS
|
| Street address |
NO.1 Beichen West Road, Chaoyang District
|
| City |
Beijing |
| ZIP/Postal code |
100101 |
| Country |
China |
| |
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| Platforms (1) |
| GPL23614 |
Streptomyces_coelicolor_M145OA_NimbleGen 12×135k array |
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| Samples (7)
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| GSM2678501 |
Global gene expression profile of Streptomyces coelicolor M145-OA at 18 h |
| GSM2678502 |
Global gene expression profile of Streptomyces coelicolor M145-OA at 24 h |
| GSM2678503 |
Global gene expression profile of Streptomyces coelicolor M145-OA at 30 h |
| GSM2678504 |
Global gene expression profile of Streptomyces coelicolor M145-OA at 36 h |
| GSM2678505 |
Global gene expression profile of Streptomyces coelicolor M145-OA at 42 h |
| GSM2678506 |
Global gene expression profile of Streptomyces coelicolor M145-OA at 48 h |
| GSM2678507 |
Global gene expression profile of Streptomyces coelicolor M145-OA at 60 h |
|
| Relations |
| BioProject |
PRJNA391411 |
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