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| Status |
Public on Jan 01, 2010 |
| Title |
Altered gene expression in zebrafish embryos exposed to tert-butylhydroquinone and 2,3,7,8-tetrachlorodibenzo-p-dioxin |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by array
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| Summary |
Oxidative stress is an important mechanism of chemical toxicity, contributing to teratogenesis as well as to cardiovascular and neurodegenerative diseases. Developing animals may be especially sensitive to chemicals causing oxidative stress. To assess the response to chemicals at different stages of development, zebrafish (Danio rerio) embryos at 1, 2, 3, 4, 5, and 6 days post-fertilization (dpf) were exposed to 0.1% dimethyl sulfoxide (DMSO), 2 nM 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), or 10 uM tert-butylhydroquinone (tBHQ) for 6 hr. Transcript abundance was assessed by real-time qRT-PCR and microarray (Agilent 22k). By microarray, 1477 probes were significantly different among the DMSO-, tBHQ-, and TCDD-treated embryos at 4 dpf. Of these, 220 were 2-fold or greater up-regulated and 108 were 2-fold or greater down-regulated by tBHQ. For TCDD, 17 probes were 2-fold or greater up-regulated and 6 were 2-fold or greater down-regulated. Genes induced by tBHQ, included genes involved in glutathione synthesis and utilization, signal transduction, and DNA damage / stress response.
Keywords: Danio rerio, TCDD, tBHQ, oxidative stress, gene expression
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| Overall design |
Separate groups of 30 embryos generated from TL adults were placed in 15 ml egg water in a 10-cm glass petri and at 1, 2, 3, 4, 5, and 6-dpf were exposed for 6 hr to 0.1% dimethyl sulfoxide (DMSO), 2 nM 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), or 10 uM tert-butylhydroquinone (tBHQ) (4 replicates per compound per time point). Embyros were frozen immediately after the 6 hr exposure and RNA isolated for microarray analysis. A Universal RNA Reference Sample was created by mixing equal amounts of total RNA from 2 replicates each from all toxicants (TCDD, tBHQ, DMSO) and timepoints (1, 2, 3, 4, 5, 6-dpf). Based on qRT-PCR results, the 4 dpf timepoint was selected for microarray analysis, in which the RNA samples (4 replicates per compound) were Cy3 labeled and co-hybridized with Cy5 labeled Universal RNA Reference Sample to generate data for analysis of variance.
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| Contributor(s) |
McArthur AG, Hahn ME, Karchner SI, Franks DG, Woodin BR, Barott KL, Cipriano MJ |
| Citation(s) |
25402455 |
| Submission date |
Jan 11, 2008 |
| Last update date |
Nov 24, 2014 |
| Contact name |
Andrew G. McArthur |
| E-mail(s) |
mcarthua@mcmaster.ca
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| Organization name |
McMaster University
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| Department |
Biochemistry & Biomedical Sciences
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| Street address |
1280 Main Street West
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| City |
Hamilton |
| State/province |
Ontario |
| ZIP/Postal code |
L8S 4K1 |
| Country |
Canada |
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| Platforms (1) |
| GPL2878 |
Agilent-013223 D. rerio (Zebrafish) Oligo Microarray G2518A Option 001 (Feature Number version) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA108351 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE10157_RAW.tar |
84.9 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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