|
| Status |
Public on Aug 23, 2017 |
| Title |
RNA-seq Analysis of Tfcp2l1ΔCP2-regulated Genes in Human ESCs |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose:The goal of this study is to analyze the candidate genes motified by Tfcp2l1ΔCP2 to regulate the human embryonic stem cell (ESC) fate through RNA-seq approach.
|
| |
|
| Overall design |
HES2 human ESCs expressing PB or PB-Tfcp2l1ΔCP2 were cultured in 10% KSR basal media supplemented with 10 ng/ml FGF2 and 10 ng/ml Activin A for two passages and then were collected by using Trizol. These samples were sent to BGI (China) company to do deep sequencing by using BGISEQ-500.
|
| |
|
| Contributor(s) |
Ye S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Aug 16, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
shoudong ye |
| Organization name |
Anhui University
|
| Department |
Cell Biology
|
| Street address |
No 111, Jiu Long Road
|
| City |
Hefei |
| ZIP/Postal code |
230601 |
| Country |
China |
| |
|
| Platforms (1) |
|
| Samples (2) |
|
| Relations |
| BioProject |
PRJNA398501 |
| SRA |
SRP115541 |
