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| Status |
Public on Oct 10, 2018 |
| Title |
Transcriptome profiling reveals anti-inflammatory activity of miR-29b in multiple myeloma associated dendritic cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Dendritic cells (DCs) are among the major components of multiple myeloma (MM)-associated bone-marrow microenvironment and are involved in MM progression, growth and chemo-resistance. We observed that after 24h co-culture with different MM cell lines, DCs downregulate miR-29b expression.
To gain insight on the effects of miR-29b, we performed a transcriptome analysis of DCs after transient transfection of a negative control or miR-29b mimics and 24h co-culture with U266 cell line.
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| Overall design |
total RNA was obtained from dendritic cells obtained from three different healthy donors, transfected with either negative control or miR-29b mimics and co-cultured for 24h with U266 cell line
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| Contributor(s) |
Botta C, Di Martino MT, Tagliaferri P, Tassone P |
| Citation(s) |
29158557 |
| Submission date |
Oct 11, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Cirino Botta |
| E-mail(s) |
cirino.botta@gmail.com
|
| Organization name |
"Annunziata" Hospital of Cosenza
|
| Department |
Hematology Unit
|
| Street address |
Via Migliori
|
| City |
Cosenza |
| ZIP/Postal code |
87100 |
| Country |
Italy |
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| Platforms (1) |
| GPL17586 |
[HTA-2_0] Affymetrix Human Transcriptome Array 2.0 [transcript (gene) version] |
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| Samples (6)
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| GSM2808487 |
dendritic cells transfected with negative control - donor 1 |
| GSM2808488 |
dendritic cells transfected with miR-29b mimics - donor 1 |
| GSM2808489 |
dendritic cells transfected with negative control - donor 2 |
| GSM2808490 |
dendritic cells transfected with miR-29b mimics - donor 2 |
| GSM2808491 |
dendritic cells transfected with negative control - donor 3 |
| GSM2808492 |
dendritic cells transfected with miR-29b mimics - donor 3 |
|
| Relations |
| BioProject |
PRJNA413954 |
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