|
| Status |
Public on Dec 05, 2017 |
| Title |
E. coli MazF does not create specialized ribosomes that translate leaderless mRNAs, but instead blocks rRNA maturation and ribosome biogenesis [Ribo-Seq] |
| Organism |
Escherichia coli |
| Experiment type |
Other
|
| Summary |
The purpose of this experiment was to assess translation of mRNA after MazF expression to determine if cleavage events resulted in a loss in ribosome footprints.
|
| |
|
| Overall design |
A + MazF sample at 5 minutes and 25 minutes after induction and an empty vector sample at 15 minutes of induction
|
| |
|
| Contributor(s) |
Culviner PH, Laub MT |
| Citation(s) |
29861158 |
| Submission date |
Nov 24, 2017 |
| Last update date |
Sep 05, 2021 |
| Contact name |
Peter Culviner |
| Organization name |
MIT
|
| Department |
Biology
|
| Lab |
Laub
|
| Street address |
31 Ames St
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL21222 |
Illumina NextSeq 500 (Escherichia coli) |
|
| Samples (3) |
|
| This SubSeries is part of SuperSeries: |
| GSE107330 |
E. coli MazF does not create specialized ribosomes that translate leaderless mRNAs, but instead blocks rRNA maturation and ribosome biogenesis |
|
| Relations |
| BioProject |
PRJNA419768 |
| SRA |
SRP125609 |
