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| Status |
Public on Aug 21, 2018 |
| Title |
Regulation of the human placental (pro)renin receptor-prorenin-angiotensin system by microRNAs |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
The placental renin-angiotensin system (RAS) is important for placentation. RAS expression is greatest in early gestation. This may be due (in part) to suppression of miRNAs that target the placental RAS, but this has never been explored.
In this study, human placental miRNAs were measured at 10–11 (early), 14–18 (mid), and 38–40 (term) weeks gestation, as well as in placentae from women with early- or late-onset preeclampsia (n=4/group), using an Agilent miRNA microarray (V19). All miRNAs showed a gestational increase and could influence the transgestational profile of the human placental RAS. Additionally, on the array, three miRNAs predicted to target the RAS (miR-892c-3p, miR-378c and miR-514-3p ) were overexpressed in placentae of late-onset preeclamptic women.
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| Overall design |
We have identified placental miRNAs that are differentially expressed in early gestation and target RAS mRNAs. At least two suppress renin production by trophoblasts. Placental miRNAs could potentially regulate placental growth and development through actions on the placental RAS.
A total of 24 primary human placental samples were used in this study, from which we were able to compare the miRNA profiles across patients with and without preeclampsia, and all across gestation. Gene expression microarray analysis was used to reveal which transcripts were differentially expressed between different gestational time points and patients with preeclampsia.
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| Contributor(s) |
Wang Y, Lumbers ER, Arthurs AL, Corbisier de Meaultsart C, Mathe A, Avery-Kjieda KA, Roberts CT, Broughton Pipkin F, Marques FZ, Morris BJ, Pringle KG |
| Citation(s) |
30060096 |
| Submission date |
Jan 30, 2018 |
| Last update date |
Aug 21, 2018 |
| Contact name |
Kirsty G Pringle |
| E-mail(s) |
kirsty.pringle@newcastle.edu.au
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| Phone |
40420329
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| Organization name |
University of Newcastle
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| Department |
HMRI
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| Lab |
Level 3 East
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| Street address |
1 Kookaburra Circuit
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| City |
Newcastle |
| State/province |
NSW |
| ZIP/Postal code |
2305 |
| Country |
Australia |
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| Platforms (1) |
| GPL18402 |
Agilent-046064 Unrestricted_Human_miRNA_V19.0_Microarray (miRNA ID version) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA432145 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE109832_RAW.tar |
203.7 Mb |
(http)(custom) |
TAR (of TXT) |
| GSE109832_miRNA_matrix.txt.gz |
56.0 Kb |
(ftp)(http) |
TXT |
| Processed data provided as supplementary file |
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