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| Status |
Public on Jul 10, 2018 |
| Title |
Genome-wide identification of protein binding sites in mammalian cells. |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
Expression profiling by high throughput sequencing
Other
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| Summary |
We report the application of genome-wide protein binding sites capture (GWPBS-Cap) technology for high-throughput profiling of protein binding sites in mammalian cells. By obtaining over 37 billion bases of sequence from NaCl washed chromatin DNA in each group, we got an unprecedented view into genome-wide binding patterns of many DNA binding proteins of Hela cells. We find that active promoters contain more PBSs with lower NaCl tolerance and our technology provides a genome-wide landscape of DNA-protein interactions.
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| |
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| Overall design |
Identification of 6 different groups of DNA-protein binding patterns in 1 cell types.
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| |
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| Contributor(s) |
Liu F, Zhang Y |
| Citation missing |
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| Submission date |
Jul 09, 2018 |
| Last update date |
Dec 16, 2019 |
| Contact name |
Liu Fenglin |
| E-mail(s) |
liufl@ioz.ac.cn
|
| Phone |
15001248122
|
| Organization name |
Chinese Academy of Sciences
|
| Street address |
Courtyard 1, Beichen West Road
|
| City |
Beijing |
| ZIP/Postal code |
100101 |
| Country |
China |
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| Platforms (2) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA480206 |
| SRA |
SRP152795 |
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