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| Status |
Public on Aug 21, 2019 |
| Title |
Transcriptional profile of injured hearts treated with the cholinesterase inhibitor pyridostigmine (PYR) at Day 5 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
It has been shown that acetylcholine has anti-inflammatory properties. The aim of this study is to investigate the role of acetylcholine in cardiac inflammation. We used the diphtheria toxin (DT)-cardiomyocyte ablation model (DTR model) (Lavine et al., 2014 - PNAS) to induce cardiac injury (DT, 2 days, 2.5µg/kg, IP). Beginning one week prior to DT injury, mice were treated with vehicle (PBS, phosphate buffered saline) or the cholinesterase inhibitor pyridostigmine (PYR, 3mg/kg, SQ). Hearts were harvested at Day 5 post-DT-injury. Here, we provide the transcriptional profile of wild-type/PBS, wild-type/PYR, DTR/PBS and DTR/PYR hearts.
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| Overall design |
mRNA sequencing profiles were generated from 6 each of wild-type/PBS, wild type/PYR, DTR/PBS and DTR/PYR hearts on Illumina HiSeq 3000 instruments.
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| Contributor(s) |
Rocha-Resende C, Matkovich SJ, Mann DL |
| Citation(s) |
31162139 |
| Submission date |
Jul 12, 2018 |
| Last update date |
Aug 21, 2019 |
| Contact name |
Scot J Matkovich |
| E-mail(s) |
smatkovich@gmail.com
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| Organization name |
Washington University School of Medicine
|
| Department |
Center for Cardiovascular Research
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| Street address |
660 S Euclid Ave, box 8086
|
| City |
St. Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
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| Platforms (1) |
| GPL21493 |
Illumina HiSeq 3000 (Mus musculus) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA480795 |
| SRA |
SRP153160 |
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