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| Status |
Public on Aug 29, 2018 |
| Title |
Effect of norepinephrine and focal adhesion kinase (FAK) knockdown in prostate cancer |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Chronic stress is associated with hormonal alterations that are known to promote cancer progression. The stress hormone norepinephrine promotes migration and metastasis of prostate cancer cells. Focal adhesion kinase (FAK) is a non-receptor protein tyrosine kinase which is phosphorylated during chronic stress or norepinephrine treatment. Here, we investigated how norepinephrine modulates the gene expression in Myc-CaP prostate cancer cell line. We also focused on the effect of FAK knockdown in norepinephrine-induced changes of the gene expression profile.
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| Overall design |
Myc-CaP cells stably transfected with shRNAs targeting FAK or negative control were treated with 10μM norepinephrine or vehicle for 24 hours. The effect on gene expression was assessed by RNA-Seq.
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| Contributor(s) |
Cheng Y, Yang Y |
| Citation(s) |
30504424 |
| Submission date |
Aug 28, 2018 |
| Last update date |
Mar 08, 2019 |
| Contact name |
Yan Cheng |
| E-mail(s) |
chengyan_cpu@hotmail.com
|
| Organization name |
University of Arkansas for Medical Sciences
|
| Department |
Internal Medicine
|
| Lab |
Fenghuang Zhan Lab
|
| Street address |
4301 W. Markham St.
|
| City |
Little Rock |
| State/province |
Arkansas |
| ZIP/Postal code |
72205 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA488279 |
| SRA |
SRP159027 |
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