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| Status |
Public on Jan 31, 2019 |
| Title |
Transcriptome analysis of C. glutamicum wildtype cells and the deletion strain ΔhrrA |
| Organism |
Corynebacterium glutamicum ATCC 13032 |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We evaluated how HrrA binding (found by ChAP-Seq) impacts the expression of individual target genes, by analyzing the transcriptome of the C. glutamicum wild type strain (ATCC 13032) as well as a ∆hrrA mutant. RNA-Seq analysis was performed prior to the addition of heme (T0) and 0.5 and 4 h after the heme pulse (in medium containing no other iron source).
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| Overall design |
Examination of a wildtype strain and a ∆hrrA strain in response to heme at three different timepoints
Please note that the *genecounts.tsv processed data was generated from both wild type and hrrA samples (per each time point) and is linked to the corresponding WT sample records.
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| Contributor(s) |
Keppel M, Viets U, Pfeifer E, Filipchyk A, Huennefeld M |
| Citation(s) |
32453397 |
| Submission date |
Oct 08, 2018 |
| Last update date |
Jul 20, 2020 |
| Contact name |
Andrei Filipchyk |
| E-mail(s) |
a.filipchyk@fz-juelich.de
|
| Phone |
+49 2461 61 2544
|
| Organization name |
FZ-Juelich
|
| Department |
IBG-1
|
| Lab |
Frunzke AG
|
| Street address |
Wilhelm-Johnen-Straße
|
| City |
Juelich |
| ZIP/Postal code |
52428 |
| Country |
Germany |
| |
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| Platforms (1) |
| GPL25416 |
Illumina MiSeq (Corynebacterium glutamicum ATCC 13032) |
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| Samples (6)
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| GSM3421586 |
Gene expression Before heme induction_WT |
| GSM3421587 |
Gene expression Before heme induction_∆hrrA_ |
| GSM3421588 |
Gene expression 0.5 hours after heme induction_WT |
| GSM3421589 |
Gene expression 0.5 hours after heme induction_∆hrrA |
| GSM3421590 |
Gene expression 4 hours after heme induction_WT |
| GSM3421591 |
Gene expression 4 hours after heme induction_∆hrrA |
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| Relations |
| BioProject |
PRJNA495033 |
| SRA |
SRP164403 |
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