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| Status |
Public on Aug 13, 2008 |
| Title |
Microarray analysis of human monocytes infected with Francisella tularensis |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Differential expression in human peripheral blood monocytes between F. novicida-infected and uninfected, and between Francisella tularensis tularensis isolate Schu S4 and uninfected.
The goal was to examine genomewide transcriptional reponses to these two strains, and identify differentially-regulated genes that may help explain the virulence of Schu S4.
Keywords: Immune Response, Human Monocytes, Bacteria, Francisella
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| Overall design |
Human monocytes were infected with the Schu S4 isolate of Francisella tularensis tularensis (n=4), with F. tularensis subspecies novicida isolate U112 (n=4) or were left uninfected (n=6). Gene expression values were calculated using the gcrma package in R and BioConductor, and limma to identify differentially expressed genes. Submitted here are expression values calculated using R 2.7.1 and BioConductor 2.2 (FreeBSD/amd64) but the original were done using R 2.6.1 and BioConductor 2.1 (FreeBSD/amd64). Twelve other chips were pooled with these 14 for preprocessing.
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| Contributor(s) |
Butchar JP, Tridandapani S |
| Citation(s) |
18698339 |
| Submission date |
Jul 14, 2008 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Jonathan P Butchar |
| E-mail(s) |
jon.butchar@osumc.edu
|
| Organization name |
The Ohio State University
|
| Department |
Internal Medicine
|
| Lab |
Tridandapani
|
| Street address |
400 W. 12th Avenue
|
| City |
Columbus |
| State/province |
OH |
| ZIP/Postal code |
43210 |
| Country |
USA |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA113801 |
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