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| Status |
Public on Nov 26, 2018 |
| Title |
Effect of high-fat diet on hepatic gene expression in SM/J mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We identified 4,356 genes with expression differences associated with a high-fat diet, with 184 genes exhibiting a sex-by-diet interaction. Dietary fat dysregulated several pathways, such as cytokine-cytokine receptor interaction, chemokine signaling, and oxidative phosphorylation.
Grant:
Funding source: American Heart Association
Grant number: 16PRE26420105
Title: The effect of maternal over-nutrition on obesity, epigenetics, and gene expression
Awarded to Madeline Keleher
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| Overall design |
We performed RNA-seq in 21 total libraries, each with two mice of the same sex and diet pooled together (There were 6 low-fat-fed female libraries, 5 libraries of high-fat-fed females, 5 libraries of low-fat-fed males, and 5 libraries of high-fat-fed males). A 1x50 single read sequencing run was done on an Illumina HiSeq 2500 machine (Illumina Inc.)
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| Citation(s) |
30526554 |
| Submission date |
Oct 19, 2018 |
| Last update date |
Mar 21, 2019 |
| Contact name |
Madeline Keleher |
| Organization name |
University of Colorado Anschutz
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| Department |
Pediatrics
|
| Street address |
12700 E 19th Ave
|
| City |
Aurora |
| State/province |
CO |
| ZIP/Postal code |
80045 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (21)
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| Relations |
| BioProject |
PRJNA497576 |
| SRA |
SRP166172 |
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