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| Status |
Public on Sep 30, 2020 |
| Title |
The effect of DZNeP-exposure on activation of hepatic stellate cells analyzed by RNA-sequencing. |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
DZNeP is the inhibitor of Ezh2 and paly negative roles on activation of hepatic stellate cells. We used RNA sequencing to identify the effective genes of DZNeP in rat HSCs. The primary rat HSCs was isolated and purified from SD rats, and cultured in DMEM culture medium with 20% FBS for 24 hours. Then the rat HSCs was administrated with DZNeP at 1μM concentration, or with similar volume of DMSO as negative control. 48 hour later, the cells were harvested and cell lysis was subjected to RNA-sequencing. This study identified that DZNeP regulated HSC activation through upregulating the expression of many key anti-fibrotic factors including Tgfβ signaling inhibitor: Bambi; cell cycle inhibitors: p21, Gadd45s; and immunomodulatory cytokine IL10.
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| Overall design |
Analysis of gene expression profiles using RNA-seq in rat primary HSCs.
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| Contributor(s) |
Jiang Y, Zhang Y, Wang H |
| Citation(s) |
33391480 |
| Submission date |
Oct 24, 2018 |
| Last update date |
Jan 07, 2021 |
| Contact name |
Yan Jiang |
| E-mail(s) |
jiangyan515@fudan.edu.cn
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| Phone |
86-021-54237158
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| Organization name |
Fudan Universtiy
|
| Department |
Institutes of biomedical sciences
|
| Street address |
Yixueyuan Rd. 138
|
| City |
Shanghai |
| ZIP/Postal code |
200032 |
| Country |
China |
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| Platforms (1) |
| GPL23945 |
Illumina HiSeq 3000 (Rattus norvegicus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA498288 |
| SRA |
SRP166769 |
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