|
| Status |
Public on Aug 07, 2019 |
| Title |
Identification of overlap between KDM3A and FOXA1 regulated transcriptome in the ER positive breast cancer cell line MCF-7 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
To interrogate the extent of overlap between KDM3A and FOXA1 regulated transcriptomes in ER-positive breast cancer cells we assessed global gene expression changes in KDM3A-depleted and FOXA1-depleted MCF-7 cells by microarray analysis using the Illumina Human HT12 Version 4 BeadChip array. We identified that 43% of the KDM3A regulated transcriptome was also regulated by FOXA1 and that 43% of the FOXA1 regulated transcriptome was also regulated by KDM3A. Genes co-regulated by KDM3A and FOXA1 were involved in ER-signalling and the cell growth. We also identified that depletion of KDM3A was required for the deposition of FOXA1 on chromatin.
|
| |
|
| Overall design |
MCF-7 cells were transfected with either a non-silencing scrambled control siRNA (siSCR) a KDM3A targeting siRNA (siKDM3A#1), or a FOXA1 targeting siRNA (siFOXA1#1) using Lipfectamine RNAiMAX (Invitrogen) to a final concentration of 25 nM in steroid depleted conditions. Cells were grown for 48 hours prior to treatment with 10 nM E2 for 6 hours before RNA extraction using TRIzol (Ambion, Life Technologies) and hybridization of triplicate samples to an Illumine HT-12 v4 BeadChip array.
|
| |
|
| Contributor(s) |
Wade MA, Jones D, Wilson L, Thomas H, Gaughan L |
| Citation(s) |
31390833 |
| Submission date |
Dec 21, 2018 |
| Last update date |
Sep 18, 2019 |
| Contact name |
Mark Wade |
| E-mail(s) |
m.wade@hull.ac.uk
|
| Organization name |
University of Hull
|
| Street address |
Room 121, Hardy Building, University of Hull, Cottingham Road
|
| City |
Hull |
| State/province |
Humberside |
| ZIP/Postal code |
HU6 7RX |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
|
| Samples (9)
|
|
| Relations |
| BioProject |
PRJNA511446 |
Less...
More...