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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jan 14, 2019 |
| Title |
The Role of CD83 in ovarian cancer cell line SKOV3 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We performed RNA-Seq of CD83 overexpression SKOV3 cells (OV_1, OV_2, OV_3), CD83 knockdown SKOV3 cells (KD_1, KD_2, KD-3), and control SKOV3 cells (NC_1, NC_2, NC_3) using BGISEQ-500 platform (BGI, China).
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| Overall design |
To initiate this study, we developed human CD83 stable overexpression and knockdown sublines of SKOV3 cells, using infection of full length CD83 lentivirus (CD83-OV) and CD83-specific lentiviral shRNA (CD83-KD), respectively. LV5 or LV3 lentivirus, containing GFP and puromycin resistant site, was served as negative control (NC). Polybrene (4 μg/ml) was used to increase the lentivirus infection efficiency. GFP-positive stable transfected cell lines were generated by two rounds of puromycin (1μg/ml) selection.
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| Contributor(s) |
Chen S, Batool A |
| Citation(s) |
32823589 |
| Submission date |
Jan 13, 2019 |
| Last update date |
Sep 08, 2020 |
| Contact name |
Su-Ren Chen |
| E-mail(s) |
chensuren@ioz.ac.cn
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| Organization name |
Institute of Zoology, Chinese Academy of Sciences
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| Department |
State Key Laboratory of Stem Cell and Reproductive Biology
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| Street address |
1Beichen West Road, Chaoyang District
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| City |
Beijing |
| ZIP/Postal code |
100101 |
| Country |
China |
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| Platforms (1) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA514904 |
| SRA |
SRP178846 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE125011_RPKM_values_for_ALL_samples.txt.gz |
14.9 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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