|
| Status |
Public on Sep 17, 2020 |
| Title |
TGF-b and Eomesodermin coordinate to maintain CD8+ regulatory T cells and limit spontaneous germinal center reaction |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We are interested in CD8+ regulatory T cells that limit germinal center reactions. We have found that in conditional knockout mice lacking both Tgfbr2 and Eomes expression in mature T cells, CD8+ regulatory T cells are severely defective. RNA-seq analysis was employed to characterize the genome-wide transcriptional changes in CD8+ regulatory T cells.
|
| |
|
| Overall design |
CD8+ regulatory T cells were FACS sorted from the spleens of 3-month old naïve WT and Tgfbr2-/-Eomes-/- mice. Total RNA was extracted and subjected to RNA-seq analysis.
|
| |
|
| Contributor(s) |
Mishra S, Ma C, Liu Y, Liao W, Lai Z, Zou Y, Chen Y, Zhang N |
| Citation(s) |
32991667 |
| Submission date |
Jan 15, 2019 |
| Last update date |
Dec 17, 2020 |
| Contact name |
YI ZOU |
| E-mail(s) |
zou@uthscsa.edu
|
| Organization name |
UTHSA-GCCRI
|
| Street address |
8403 Floyd Curl Drive
|
| City |
SAN ANTONIO |
| State/province |
TX |
| ZIP/Postal code |
78229 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL21493 |
Illumina HiSeq 3000 (Mus musculus) |
|
| Samples (2) |
|
| Relations |
| BioProject |
PRJNA515265 |
| SRA |
SRP179612 |
