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| Status |
Public on Jan 25, 2019 |
| Title |
A HOTAIR regulatory element modulates glioma cell sensitivity to temozolomide through long-range regulation of multiple target genes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
Temozolomide (TMZ) is a frequently used chemotherapy for glioma; however, chemoresistance is a major problem limiting its effectiveness. Thus knowledge of mechanisms underlying this outcome could improve patient prognosis. Here, we report that deletion of a regulatory element in the HOTAIR locus increases glioma cell sensitivity to TMZ and alters transcription of multiple genes. Analysis of a combination of RNA-seq, Capture HiC and patient survival data suggests that CALCOCO1 and ZC3H10 are target genes repressed by the HOTAIR regulatory element and that both function in regulating glioma cell sensitivity to TMZ. Rescue experiments and TAD analysis based on HiC data confirmed this hypothesis. We propose a new regulatory mechanism governing glioma cell TMZ sensitivity.
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| Overall design |
BioProject: PRJNA516515. SRA Study accession:SRP181265 .
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| Contributor(s) |
Zhang L, Lu W |
| Citation(s) |
31953347 |
| BioProject |
PRJNA516515 |
| Submission date |
Jan 24, 2019 |
| Last update date |
Aug 10, 2021 |
| Contact name |
Bohan Chen |
| E-mail(s) |
bhchen1@sina.cn
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| Organization name |
Nankai University
|
| Street address |
NO.94 Weijin Road
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| City |
Tianjin |
| ZIP/Postal code |
300071 |
| Country |
China |
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| Platforms (2) |
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| Samples (10)
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| Relations |
| SRA |
SRP181265 |
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