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| Status |
Public on Jun 07, 2019 |
| Title |
Chromatin conformation remains stable upon extensive transcriptional changes driven by heat shock [K562] |
| Organism |
Homo sapiens |
| Experiment type |
Other
|
| Summary |
Heat shock (HS) causes dramatic and rapid changes in transcription of thousands of genes in metazoans. We have used insitu Hi-C in Drosophila S2 and human K562 cells to determine the effects of HS upon chromatin conformation. Our study shows that topologically associated domains (TADs), compartments and distal regulatory interactions remain unchanged upon HS.
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| Overall design |
Investigate changes in chromatin conformation upon acute HS in Drosophila S2 and human K562 cells by insitu Hi-C. Two biological replicates were done for Non heat shock (NHS) or HS conditions in each organism.
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| Contributor(s) |
Ray J, Munn PR, Vihervaara A, Ozer A, Danko CG, Lis JT |
| Citation(s) |
31506350 |
| Submission date |
May 06, 2019 |
| Last update date |
Sep 16, 2019 |
| Contact name |
Charles Danko |
| E-mail(s) |
dankoc@gmail.com
|
| Organization name |
Cornell University
|
| Department |
Computational Biology
|
| Lab |
Danko Lab
|
| Street address |
235 Hungerford Drive
|
| City |
Ithaca |
| State/province |
NY |
| ZIP/Postal code |
14850 |
| Country |
USA |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (4)
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| GSM3753207 |
Biosample_8606_4132_58665_HYL2CBGX2_K562_InSituHiC_HS_1_CGATGT |
| GSM3753208 |
Biosample_8606_4132_58667_HYL2CBGX2_K562_InSituHiC_HS_2_GCCAAT |
| GSM3753209 |
Biosample_8606_4132_58664_HYL2CBGX2_K562_InSituHiC_NHS_1_ATCACG |
| GSM3753210 |
Biosample_8606_4132_58666_HYL2CBGX2_K562_InSituHiC_NHS_2_ACAGTG |
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| This SubSeries is part of SuperSeries: |
| GSE130778 |
Chromatin conformation remains stable upon extensive transcriptional changes driven by heat shock |
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| Relations |
| BioProject |
PRJNA541309 |
| SRA |
SRP195614 |
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