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Series GSE13196 Query DataSets for GSE13196
Status Public on May 01, 2009
Title Expression data from zebrafish pineal gland
Organism Danio rerio
Experiment type Expression profiling by array
Summary Microarray data allowed detection of genes that are highly expressed in the pineal gland.

Keywords: tissue expression pattern
 
Overall design Adult Tg(aanat2:EGFP)Y8 transgenic zebrafish in which EGFP marks the pineal gland were used for RNA extraction and hybridization on Affymetrix microarrays. Fish were anesthetized in 1.5 mM Tricane (Sigma) and sacrificed by decapitation, and pineal glands were removed under a fluorescent dissecting microscope. Since the pineal gland is a clock-containing organ and levels of certain transcripts may vary throughout the circadian cycle, glands were collected throughout the 24-hr cycle at 4 hr intervals. During the 24-hr cycle the fish were either maintained in a 12-h light/12-h dark cycle (LD) or kept in constant darkness (DD). 12 pineal glands were collected and pooled at each time point at each light condition, and total RNA was extracted (RNeasy, Qiagen).
 
Contributor(s) Gothilf Y
Citation(s) 19147662
Submission date Oct 14, 2008
Last update date Jan 25, 2018
Contact name Yoav Gothilf
E-mail(s) YoavG@tauex.tau.ac.il
Phone +972-3-640-6329
Fax +972-3-640-6329
Organization name Tel Aviv University
Department Neurobiology, Faculty of Life Sciences
Lab Gothilf
Street address Sherman Building, Room 405
City Tel Aviv
ZIP/Postal code 69978
Country Israel
 
Platforms (1)
GPL1319 [Zebrafish] Affymetrix Zebrafish Genome Array
Samples (14)
GSM333303 Pineal_DD_ZT2
GSM333304 Pineal_DD_ZT6
GSM333305 Pineal_DD_ZT10
Relations
BioProject PRJNA109491

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE13196_RAW.tar 36.5 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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