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| Status |
Public on Jan 20, 2020 |
| Title |
Analysis of PDCD4 knockdown effects by high throughput sequencing of a human epithelial cell line |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Other
|
| Summary |
Purpose: examination of gene expression and translation changes in immortalized human epithelial cells (hTERT-RPE-1) induced by PDCD4 siRNA-knockdown.
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| Overall design |
Knockdown induction: hTERT-RPE-1 cells were transfected either with PDCD4-siRNA or control-siRNA (100 nM) and incubated for 24 h. Induced effects were analyzed by ribosome profiling and RNA-seq in triplicate (IlluminaSequencing).
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| |
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| Contributor(s) |
Haas A, Klempnauer K, Nilges BS, Leidel SA |
| Citation(s) |
32066800 |
| Submission date |
Oct 07, 2019 |
| Last update date |
Mar 02, 2020 |
| Contact name |
Astrid Haas |
| Organization name |
WWU Münster
|
| Department |
Biochemistry
|
| Street address |
Wilhelm-Klemm-Str.2
|
| City |
Münster |
| ZIP/Postal code |
48149 |
| Country |
Germany |
| |
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| Platforms (1) |
| GPL15456 |
Illumina HiScanSQ (Homo sapiens) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA576260 |
| SRA |
SRP224660 |
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