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| Status |
Public on Sep 30, 2020 |
| Title |
Direct induction of retinal ganglion cell-like neurons |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Retinal ganglion cells (RGCs) are the projection neurons in the retina that connect the visual sensing tissue to the brain. We found that Ascl1/Brn3b/Isl1 transcription factor combination can quickly and efficiently reprogramming mouse embryonic fibroblasts (MEFs) into retinal ganglion cell-like neurons (iRGCs). Using RNA-seq, we analyzed the transcriptomes of MEFs infected with Ascl1/Brn3b/Isl1-overexpressing viruses on day 2 or day 7 of reprogramming, or the final iRGCs on day 13 of reprogramming.
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| Overall design |
MEFs infected with Ascl1/Brn3b/Isl1-overexpressing lentiviruses were FACS sorted based on GFP expression (linked to the end of Ascl1/Brn3b/Isl1 through 2A peptides), and processed for RNA-seq library construction and sequencing.
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| Contributor(s) |
Chen S |
| Citation(s) |
33096050 |
| Submission date |
Nov 19, 2019 |
| Last update date |
Nov 24, 2020 |
| Contact name |
SHUYI CHEN |
| E-mail(s) |
chenshy23@mail.sysu.edu.cn
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| Organization name |
Zhongshan Ophthalmic Center, SYSU
|
| Department |
State Key Laboratory of Ophthalmology
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| Street address |
54 S. Xianlie Rd
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| City |
Guangzhou |
| State/province |
Guangdong |
| ZIP/Postal code |
510060 |
| Country |
China |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (23)
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| Relations |
| BioProject |
PRJNA590512 |
| SRA |
SRP230635 |
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