|
| Status |
Public on Jul 23, 2021 |
| Title |
Analysis of RNA localization in nucleolin deficient axons |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We previously showed that the RNA binding protein Nucleolin is localized to axons of DRG sensory neurons by interaction with the molecular motor Kinesin-1 (Kif5A) and subsequently localizes importin beta1 mRNA there (Perry et al., 2016). In this work, we created a mouse with a heterozygous deletion of the domain in nucleolin responsible for the Kif5A interaction and showed that nucelolin protein levels are reduced in the axons of dorsal root ganglia neurons in this mouse model. The goal of this study was to identify RNAs who's axonal localization is affected by this perturbation.
|
| |
|
| Overall design |
Total RNA from the neurite or soma compartments of dorsal root ganalia neurons cultured on modified Boyden chambers was collected and analysed by RNA sequencing. We repeated this in 3 independent biological repeats.
|
| |
|
| Contributor(s) |
Doron-Mandel E, Abraham O, Koppel I, Kawaguchi R, Coppola G, Fainzilber M |
| Citation(s) |
34515347 |
| Submission date |
Dec 16, 2019 |
| Last update date |
Oct 22, 2021 |
| Contact name |
Riki Kawaguchi |
| E-mail(s) |
rkawaguchi@mednet.ucla.edu
|
| Phone |
4244424783
|
| Organization name |
University of California Los Angeles
|
| Department |
Department of Neurology and Department of Psychiatry
|
| Lab |
Informatics Center for Neurogenetics and Neurogenomics (ICNN).
|
| Street address |
760 Westwood Plaza, Room 37-420
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095-1759 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
|
| Samples (12)
|
|
| This SubSeries is part of SuperSeries: |
| GSE142576 |
GAR-Driven Subcellular Localization of the Growth Controlling RNA Binding Protein Nucleolin |
|
| Relations |
| BioProject |
PRJNA595902 |
| SRA |
SRP237768 |
Less...
More...