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| Status |
Public on Jun 02, 2020 |
| Title |
Genome-wide analyses of chromatin interactions after the loss of Pol I, Pol II and Pol III [4C-seq] |
| Organism |
Mus musculus |
| Experiment type |
Other
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| Summary |
We analyzed Hi-C, HiChIP, Ocean-C, RNA-seq, ATAC-seq, ChIP-seq and 4C-seq data to provide the most comprehensive evidence to date to demonstrate that transcription plays a marginal role in organizing the 3D genome in mammalian cells: 1) degraded Pol I, Pol II and Pol III proteins in mESCs, and showed their loss results in little or no changes of global 3D chromatin structures for the first time; 2) selected RNA polymerases high abundance binding sites-associated interactions and found they still persist after the degradation; 3) generated higher resolution chromatin interaction maps and revealed that transcription inhibition mildly alters small loop domains; 4) identified Pol II bound but CTCF and Cohesin unbound loops and disclosed that they are largely resistant to transcription inhibition
Interestingly, we found that Pol II depletion for a longer time significantly affects the chromatin accessibility and Cohesin occupancy, suggesting RNA polymerases are capable of affecting the 3D genome indirectly. So, the direct and indirect effects of transcription inhibition explain the previous confusing effects on the 3D genome. We conclude that Pol I, Pol II, and Pol III loss only mildly alter chromatin interactions in mammalian cells, suggesting the 3D chromatin structures are preestablished and relatively stable.
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| Overall design |
We have finished the Hi-C, HiChIP, Ocean-C, RNA-seq, ATAC-seq, ChIP-seq and 4C-seq to investigate the roles of Pol I, Pol II and Pol III in 3D genome organization and transcription. The V6.5 mouse ES (mES) cell line was used to perform all the high-throughput analysis. Degron mES cells were treated with 1 μg/ml Doxycycline for different hours.
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| Contributor(s) |
Jiang Y, Huang J, Lun K, Li B |
| Citation(s) |
32616013 |
| BioProject |
PRJNA607400 |
| Submission date |
Feb 24, 2020 |
| Last update date |
Jul 13, 2020 |
| Contact name |
Xiong Ji |
| E-mail(s) |
xiongji@pku.edu.cn
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| Organization name |
Peking University
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| Department |
School of Life Sciences
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| Lab |
Jilab
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| Street address |
Haidian District, Beijing Summer Palace Road No. 5
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| City |
Beijing |
| ZIP/Postal code |
100871 |
| Country |
China |
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| Platforms (1) |
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| Samples (14)
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| This SubSeries is part of SuperSeries: |
| GSE145874 |
Genome-wide analyses of chromatin interactions after the loss of Pol I, Pol II and Pol III |
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| Relations |
| SRA |
SRP250063 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE145784_RAW.tar |
1.2 Gb |
(http)(custom) |
TAR (of BW) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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