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| Status |
Public on Jul 10, 2020 |
| Title |
Analysis of lung transcriptomic changes following inhibition of LTβR-signalling in cigarette smoke exposed mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
How LTβR-signalling drives chronic tissue damage particularly in the lung, which mechanisms regulate this process, and whether LTβR-blockade might be of therapeutic value has remained unclear. To study the mechanisms underlying LTβR-inhibition, a transcriptional analysis was performed on lung tissue from B6 mice exposed to cigarette smoke for 6 months and treated therapeutically with LTβR-Ig from 4 to 6 months compared to mice exposed to cigarette smoke for 6 months and treated with control Ig from 4 to 6 months and filtered air control. Single-cell RNA-Seq identified 24 distinct cell populations across >20,000 cells in lungs with distinct changes occurring upon cigarette smoke exposure and LTβR-Ig treatment.
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| Overall design |
Single-cell transcriptomics experiment after cigarette smoke exposure and drug treatment.
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| Contributor(s) |
Conlon TM, John-Schuster G, Ansari M, Strunz M, Theis FJ, Schiller HB, Heikenwalder M, Yildirim AO |
| Citation(s) |
33149305, 35031603 |
| Submission date |
Jun 02, 2020 |
| Last update date |
Feb 02, 2022 |
| Contact name |
Meshal Ansari |
| Organization name |
Helmholtz Zentrum Munich
|
| Street address |
Ingolstädter Landstrasse 1
|
| City |
Neuherberg |
| State/province |
Bavaria |
| ZIP/Postal code |
85764 |
| Country |
Germany |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (13)
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| Relations |
| BioProject |
PRJNA636811 |
| SRA |
SRP265656 |
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