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| Status |
Public on Jan 24, 2021 |
| Title |
Comparative transcriptome analysis of Brucella canis MucR mutant strain and its parent strain RM6/66 |
| Organism |
Brucella canis |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose:To uncover the related mechanisms underlie virulence attenuation of Brucella canis MucR mutant strain. Methods:Three Brucella canis RM6/66 strains and three Brucella canis ΔmucR strains were grown in TSB at 37℃ until the log phase was reached, total RNA was isolated using the TRIzol according to the manufacturer’s instructions.The sequencing library of each RNA sample was prepared by using NEB Next Ultra Directional RNA Library Prep Kit for Illumina as recommended by the manufacturer. An Illumina platform was used to perform the transcriptome sequencing. Results: The results revealed that expressions of 694 genes were significantly different between RM6/66 and ΔmucR. Data analysis showed that in the COG term, the different expressed genes involved in translation, ribosomal structure and biogenesis, signal transduction mechanisms, energy production and conversion, intracellular trafficking, secretion, and vesicular transport, and extracellular structures were significantly affected. Pathway enrichment analysis indicated that the genes involved in ribosome, oxidative phosphorylation, aminoacyl-tRNA biosynthesis and protein export were significantly enriched.
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| Overall design |
mRNA profiles of Three Brucella canis RM6/66 strains and three Brucella canis ΔmucR strains were generated by deep sequencing using HiSeq 4000 platform
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| Contributor(s) |
Ding J, Dong H |
| Citation(s) |
34250056 |
| Submission date |
Aug 05, 2020 |
| Last update date |
Jul 15, 2021 |
| Contact name |
Hao Dong |
| E-mail(s) |
houpengwei@novelbio.com
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| Phone |
+86-10-59198917
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| Organization name |
China Animal Disease Control Center
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| Street address |
Tiangui road NO.17
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| City |
Beijing |
| ZIP/Postal code |
102618 |
| Country |
China |
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| Platforms (1) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA655476 |
| SRA |
SRP276107 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE155748_all.counts.exp.txt.gz |
50.1 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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