|
| Status |
Public on Nov 10, 2020 |
| Title |
Capturing cardiogenesis in gastruloids |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Organoids are powerful models for studying tissue development, physiology, and disease. However, current culture systems disrupt the inductive tissue-tissue interactions needed for the complex morphogenetic processes of native organogenesis. Here we show that mouse embryonic stem cells (mESCs) can be coaxed to robustly undergo fundamental steps of early heart organogenesis with an in vivo-like spatiotemporal fidelity. These axially patterned embryonic organoids (gastruloids) mimic embryonic development, and support the generation of cardiovascular progenitors, including first and second heart fields. The cardiac progenitors self-organize into an anterior domain reminiscent of a cardiac crescent before forming a beating cardiac tissue near a putative primitive gut-like tube, from which it is separated by an endocardial-like layer. These findings unveil the surprising morphogenetic potential of mESCs to execute key aspects of organogenesis through the coordinated development of multiple tissues. This platform could be an excellent tool for studying heart development in unprecedented detail and throughput.
For data exploration, execute in R-Studio Explore_Data.R in
https://github.com/nbroguiere/Cardiac_Gastruloids
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| |
|
| Overall design |
10x single-cell RNA-seq of gastruloids at day 4, 5, 6 and 7, in duplicates.
|
| |
|
| Contributor(s) |
Rossi G, Broguiere N, Lutolf M |
| Citation(s) |
33176168, 35927563 |
| Submission date |
Oct 05, 2020 |
| Last update date |
Aug 16, 2022 |
| Contact name |
Nicolas Broguiere |
| E-mail(s) |
nicolas.broguiere@epfl.ch
|
| Organization name |
EPFL
|
| Department |
Sciences de la vie
|
| Lab |
Laboratory of Stem Cell Bioengineering
|
| Street address |
AI 1208 Station 15
|
| City |
Lausanne |
| ZIP/Postal code |
1015 |
| Country |
Switzerland |
| |
|
| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA667329 |
| SRA |
SRP286349 |
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