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| Status |
Public on Nov 17, 2020 |
| Title |
Next Generation Sequencing of lung homogenate mRNA from 8-12 week male C57BL/6 mice following Sham, Carbon Black, Ozone, or Carbon Black-Ozone Co-exposure. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
While investigation of a single inhaled toxicant may be useful, the additive effects of toxicants interacting within the body can only be modelled through co-exposure. The purpose of this study is to understand how inhalation of carbon black and ozone alters the transcriptome, and if co-exposure incites unique, genome-wide changes in the lungs.
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| Overall design |
C57BL/6 mice (8-12 weeks old) were exposed to carbon black (10 mg/m3) and/or ozone (2 ppm) for a duration of (3 hours) over a single exposure (Day 1) or four independent exposures (Day 4). Lungs were removed, RNA isolated, and mRNA sequencing performed using the Illumina HiSeq. Libraries were built with the TruSeq® Stranded mRNA Library Prep Kit (Illumina, San Diego, CA) and run on the HiSeq 2500 (Illumina) in 51bp paired end reads. Fastq files were mapped and quantified using the mapping-based mode of Salmon 1.1.0.
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| Contributor(s) |
Hathaway QA, Hussain S, Hollander JM |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Nov 16, 2020 |
| Last update date |
Nov 17, 2020 |
| Contact name |
Quincy Alexander Hathaway |
| E-mail(s) |
qahathaway@mix.wvu.edu
|
| Phone |
724-255-4637
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| Organization name |
West Virginia University
|
| Department |
Exercise Physiology
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| Lab |
Hollander Lab
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| Street address |
1 Medical Center Road
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| City |
Morgantown |
| State/province |
WV |
| ZIP/Postal code |
26505 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (28)
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| Relations |
| BioProject |
PRJNA678659 |
| SRA |
SRP292708 |
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