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| Status |
Public on Oct 29, 2022 |
| Title |
RNA-Seq of injured mouse hearts depleted for myofibroblast Yap [I] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
A myofibroblast specific tamoxifen inducible Cre was used to assess the contribution of Yap in the mouse heart after myocardial infarction
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| Overall design |
We implemented a Cre-lox system whereby inducible Cre expressed under the myofibroblast specific Periostin promoter is crossed with Yap floxed animals, facilitating inducible depletion of Yap. Mice were siblings with floxed Yap alleles and either a copy of MerCreMer under the endogenous periostin promote or wildtype Periostin. Permenant ligation of the left anterior descending artery or sham surgeries were conducted. Mice were administered tamoxifen citrate diet ad libitum immediately after surgery to promote excession of myofibroblast yap and left ventricles were collected 4 days post injury.
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| Contributor(s) |
Flinn MA, O'Meara CC, Patterson M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Dec 15, 2020 |
| Last update date |
Oct 31, 2022 |
| Contact name |
Michael Andrew Flinn |
| Organization name |
Medical College of Wisconsin
|
| Department |
Physiology
|
| Lab |
O'Meara Lab
|
| Street address |
8701 W Watertown Plank Rd
|
| City |
Wauwatosa |
| State/province |
WI |
| ZIP/Postal code |
53226 |
| Country |
USA |
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| Platforms (1) |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE163617 |
RNA-seq of injured mouse hearts depleted for myofibroblast Yap or Yap and Wwtr1 |
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| Relations |
| BioProject |
PRJNA685438 |
| SRA |
SRP298047 |
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