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| Status |
Public on Sep 01, 2021 |
| Title |
Human skeletal muscle transcriptome in response to high-intensity interval training |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Skeletal muscle tissue is a highly adaptable tissue, responding to the specific demands it is subjected to. High-intensity interval training (HIIT) has been shown to generate similar, or even greater, molecular changes in skeletal muscle as that of constant load longer-lasting endurance-type training at moderate intensities. Despite shorter exposure times, the higher intensity provided by HIIT training leads to greater metabolic perturbations and thereby larger improvements in mitochondrial content and maximal oxygen uptake. During a period of regular exercise training, the performance improvements follow a non-linear pattern with a relatively faster pace initially and a gradual ‘plateauing-off’ after weeks and months. It is believed that this ‘plateau effect’ is due to a blunting of the molecular responses to acute exercise with exercise training. In the present study we utilised an explorative global transcriptomic approach to investigate the phenomenon of transcriptional-level blunting of the acute exercise response in human skeletal muscle over the course of a three-week HIIT intervention. We hypothesize that the blunting of transcription at this time-point is specific to certain pathways, including metabolic regulation and that these genes have communal transcriptional regulation.
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| Overall design |
Eleven healthy men with mean age of 24.8 years and VO2peak of 51.9 ± 1.9 ml O2x kg-1 x min-1 performed nine sessions of HIIT (10 x 4 minutes of cycling at 91 % of HRmax) over three weeks. Before and 3 h after the 1st and 9th exercise bout, skeletal muscle biopsies were obtained, and RNA sequencing was performed.
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| Contributor(s) |
Rullman E, Lovric A |
| Citation(s) |
35482326, 38561245 |
| Submission date |
Dec 16, 2020 |
| Last update date |
Apr 17, 2024 |
| Contact name |
Eric Rullman |
| E-mail(s) |
eric.rullman@gmail.com
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| Phone |
+46858586777
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| Organization name |
Karolinska Institutet
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| Department |
Laboratory Medicine
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| Lab |
Clinical Physiology
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| Street address |
C1:82 Karolinska University Hospital, Huddinge
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| City |
Stockholm |
| ZIP/Postal code |
14186 |
| Country |
Sweden |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (41)
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| GSM4977400 |
Subject A, Post-exercise, First session A_T1_2 |
| GSM4977401 |
Subject A, Pre-exercise, First session A_T1_1 |
| GSM4977402 |
Subject A, Post-exercise, Ninth/Last session A_T9_2 |
| GSM4977403 |
Subject A, Pre-exercise, Ninth/Last session A_T9_1 |
| GSM4977404 |
Subject B, Post-exercise, First session B_T1_2 |
| GSM4977405 |
Subject B, Pre-exercise, First session B_T1_1 |
| GSM4977406 |
Subject B, Post-exercise, Ninth/Last session B_T9_2 |
| GSM4977407 |
Subject B, Pre-exercise, Ninth/Last session B_T9_1 |
| GSM4977408 |
Subject C, Post-exercise, First session C_T1_2 |
| GSM4977409 |
Subject C, Pre-exercise, First session C_T1_1 |
| GSM4977410 |
Subject C, Post-exercise, Ninth/Last session C_T9_2 |
| GSM4977411 |
Subject C, Pre-exercise, Ninth/Last session C_T9_1 |
| GSM4977412 |
Subject D, Post-exercise, First session D_T1_2 |
| GSM4977413 |
Subject D, Pre-exercise, First session D_T1_1 |
| GSM4977414 |
Subject D, Post-exercise, Ninth/Last session D_T9_2 |
| GSM4977415 |
Subject D, Pre-exercise, Ninth/Last session D_T9_1 |
| GSM4977416 |
Subject E, Post-exercise, First session E_T1_2 |
| GSM4977417 |
Subject E, Pre-exercise, First session E_T1_1 |
| GSM4977418 |
Subject E, Post-exercise, Ninth/Last session E_T9_2 |
| GSM4977419 |
Subject E, Pre-exercise, Ninth/Last session E_T9_1 |
| GSM4977420 |
Subject F, Post-exercise, First session F_T1_2 |
| GSM4977421 |
Subject F, Pre-exercise, First session F_T1_1 |
| GSM4977422 |
Subject F, Post-exercise, Ninth/Last session F_T9_2 |
| GSM4977423 |
Subject F, Pre-exercise, Ninth/Last session F_T9_1 |
| GSM4977424 |
Subject G, Post-exercise, First session G_T1_2 |
| GSM4977425 |
Subject G, Pre-exercise, First session G_T1_1 |
| GSM4977426 |
Subject G, Post-exercise, Ninth/Last session G_T9_2 |
| GSM4977427 |
Subject H, Post-exercise, First session H_T1_2 |
| GSM4977428 |
Subject H, Pre-exercise, First session H_T1_1 |
| GSM4977429 |
Subject H, Post-exercise, Ninth/Last session H_T9_2 |
| GSM4977430 |
Subject H, Pre-exercise, Ninth/Last session H_T9_1 |
| GSM4977431 |
Subject I, Post-exercise, First session I_T1_2 |
| GSM4977432 |
Subject I, Pre-exercise, First session I_T1_1 |
| GSM4977433 |
Subject I, Post-exercise, Ninth/Last session I_T9_2 |
| GSM4977434 |
Subject I, Pre-exercise, Ninth/Last session I_T9_1 |
| GSM4977435 |
Subject J, Post-exercise, First session J_T1_2 |
| GSM4977436 |
Subject J, Pre-exercise, First session J_T1_1 |
| GSM4977437 |
Subject J, Pre-exercise, Ninth/Last session J_T9_1 |
| GSM4977438 |
Subject K, Post-exercise, First session K_T1_2 |
| GSM4977439 |
Subject K, Post-exercise, Ninth/Last session K_T9_2 |
| GSM4977440 |
Subject K, Pre-exercise, Ninth/Last session K_T9_1 |
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| Relations |
| BioProject |
PRJNA685799 |
| SRA |
SRP298189 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE163356_counts.tsv.gz |
2.5 Mb |
(ftp)(http) |
TSV |
| GSE163356_logCPM.tsv.gz |
2.5 Mb |
(ftp)(http) |
TSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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