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| Status |
Public on Jan 16, 2021 |
| Title |
Single-Cell Trajectories of Melanoma Cell Resistance to Targeted Treatment |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In order to identify mechanisms of melanoma treatment resistance, we applied micorofluidic scRNA-Seq to the transcripomes of a total of about 31,000 single cells obtained form A375 melanoma cell line untreated/sensitive or treated with BRAF inhibitor vemurafenib, either alone or in combination with MEK1 inihibitor cobimetinib or trametinib. Analysis by dimensionality reduction software tSNE and SOM identified a clonal heterogeneity clearly seperating all 4 possible treatment conditions.
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| |
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| Overall design |
scRNA-Seq of BRAF-mutant A375 cells in 4 treatment conditions: untreated/sensitive cells, cells resistant to vemurafenib, cells double-resistant to vemurafenib and cobimetinib, cells double-resistant to vemurafenib and trametinib
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| |
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| Contributor(s) |
Schmidt M, Mortensen S, Loeffler-Wirth H, Kosnopfel C, Krohn K, Binder H, Kunz M |
| Citation(s) |
34591417 |
| Submission date |
Jan 15, 2021 |
| Last update date |
Jan 24, 2023 |
| Contact name |
Maria Schmidt |
| E-mail(s) |
schmidt@izbi.uni-leipzig.de
|
| Organization name |
IZBI
|
| Street address |
Haertelstraße 16-18
|
| City |
Leipzig |
| ZIP/Postal code |
04107 |
| Country |
Germany |
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| Platforms (1) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA692421 |
| SRA |
SRP301922 |
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