|
Status |
Public on Oct 02, 2009 |
Title |
Expression data from hESCs differentiated in the presence or absence of nicotinamide |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
|
Summary |
Human ESCs are pluripotent cells that have the capacity of self renewal for a prolonged period in vitro, and can differentiate into derivatives of all three primary germ layers: endoderm, mesoderm and ectoderm. Human ESCs are responsive to a wide range of factors in vitro that can direct their differentiation into specific cell types. We analyzed the effect of nicotinamide (NIC) on differentiation of hESCs in vitro. CEL file for GSM424319 is unavailable.
|
|
|
Overall design |
hESCs were cultured as free-floating clusters in the presence of 10% FCS for 4 weeks in the presence or absence of nicotinamide.
|
|
|
Contributor(s) |
Ben-Shushan E, Rechavi G |
Citation(s) |
19796620 |
Submission date |
Jul 01, 2009 |
Last update date |
Dec 06, 2018 |
Contact name |
Reba Condiotti |
E-mail(s) |
reba@hadassah.org.il
|
Organization name |
Hadassah Medical Center
|
Department |
Goldyne Savad Institute of Gene Therapy
|
Street address |
P.O.B. 12000
|
City |
Jerusalem |
ZIP/Postal code |
91120 |
Country |
Israel |
|
|
Platforms (1) |
GPL571 |
[HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array |
|
Samples (6)
|
GSM424314 |
H50 untreated hESCs, biological rep1 |
GSM424315 |
H51 untreated hESCs, biological rep2 |
GSM424316 |
H52 untreated hESCs, biological rep3 |
GSM424317 |
H53 NIC-treated hESCs, biological rep1 |
GSM424318 |
H54 NIC-treated hESCs, biological rep2 |
GSM424319 |
H55 NIC-treated hESCs, biological rep3 |
|
Relations |
BioProject |
PRJNA117627 |