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| Status |
Public on Feb 09, 2022 |
| Title |
Single-cell Transcriptome Analysis Revealed that EGFR Mutation Leads to a Suppressive Tumor Immune Microenvironment in Lung Adenocarcinoma |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We analyzed 40,799 single cells from nine samples and found that in EGFR-mutant LUAD, tumor cells generated an immunosuppressive microenvironment by releasing cytokines to attract suppressive immune cells such as myeloid-derived suppressor cells (MDSCs) directly or indirectly by interacting with macrophages, dendritic cells, or other cell types. By contrast, EGFR wild-type LUAD had a greater ability to recruit immunocompetent T cells such as TRMs and Th1 cells, thus driving a proinflammatory microenvironment.
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| |
|
| Overall design |
To evaluate the cellular landscape of the TME of lung adenocarcinoma based on EGFR mutation status, we collected nine tumor samples from eight patients with treatment-naïve LUAD for scRNA-seq and bioinformatic analyses.
|
| Web link |
https://0-jitc-bmj-com.brum.beds.ac.uk/content/10/2/e003534
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| |
|
| Contributor(s) |
Zhou Q, Yang L |
| Citation(s) |
35140113 |
| Submission date |
Mar 30, 2021 |
| Last update date |
Mar 04, 2022 |
| Contact name |
Qing Zhou |
| E-mail(s) |
gzzhouqing@126.com
|
| Organization name |
Guangdong Lung Cancer Institute, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China
|
| Street address |
106#，ZHONG SHAN ER ROAD
|
| City |
Guangzhou |
| State/province |
Guangdong |
| ZIP/Postal code |
510010 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (9)
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|
| Relations |
| BioProject |
PRJNA718546 |
| SRA |
SRP312733 |
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