|
| Status |
Public on Jul 17, 2009 |
| Title |
Pseudomonas aeruginosa PA14, pqsE and PQS |
| Organism |
Pseudomonas aeruginosa |
| Experiment type |
Expression profiling by array
|
| Summary |
The Pseudomonas aeruginosa MvfR-dependent QS regulatory pathway controls the expression of key virulence genes; and is activated via the extracellular signals 4-hydroxy-2-heptylquinoline (HHQ) and 3,4-dihydroxy-2-heptylquinoline (PQS). Our findings reveal a multi-layered homeostatic regulation of PQS and HHQ and the MvfR regulon component PqsE which is a key mediator in orchestrating this homeostatic regulation.
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| Overall design |
We assessed the expression profile of pqsE mutant in contrast to constitutive expression of PqsE from PA14 and its mvfR mutant. In addition we tested the effect of addition of 20ug/ml PQS on mvfR mutant expressing PqsE
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| Contributor(s) |
Hazan R, He J, Xiao G, Rahme LG |
| Citation(s) |
20300606 |
| Submission date |
Jul 15, 2009 |
| Last update date |
Nov 08, 2019 |
| Contact name |
Laurence G Rahme |
| E-mail(s) |
rahme@molbio.mgh.harvard.edu
|
| Phone |
617-724-5003
|
| Fax |
617-724-8558
|
| Organization name |
Massachusetts General Hospital
|
| Department |
Surgery
|
| Street address |
50 Blosson St, Thier 340
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02114 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL84 |
[Pae_G1a] Affymetrix Pseudomonas aeruginosa Array |
|
| Samples (15)
|
| GSM428016 |
PA14 OD3 rep1 |
| GSM428018 |
PA14 OD3 rep2 |
| GSM428019 |
PA14 OD3 rep3 |
| GSM428020 |
PA14 constitutively expressing PqsE from a plasmid rep1 |
| GSM428021 |
PA14 constitutively expressing PqsE from a plasmid rep2 |
| GSM428022 |
PA14 constitutively expressing PqsE from a plasmid rep3 |
| GSM428023 |
mvfR mutant constitutively expressing PqsE from a plasmid rep1 |
| GSM428032 |
mvfR mutant constitutively expressing PqsE from a plasmid rep2 |
| GSM428033 |
mvfR mutant constitutively expressing PqsE from a plasmid rep3 |
| GSM428034 |
PQS treated mvfR mutant constitutively expressing PqsE from a plasmid rep1 |
| GSM428035 |
PQS treated mvfR mutant constitutively expressing PqsE from a plasmid rep2 |
| GSM428036 |
PQS treated mvfR mutant constitutively expressing PqsE from a plasmid rep3 |
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| This SubSeries is part of SuperSeries: |
| GSE17147 |
Pseudomonas aeruginosa PA14 MvfR-dependent QS regulatory pathway |
|
| Relations |
| BioProject |
PRJNA123657 |
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