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| Status |
Public on Apr 07, 2021 |
| Title |
Natural Variation in the Fitness Consequences of Gene Amplification in Wild Saccharomyces cerevisiae Isolates [RNA-seq] |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We used RNA-Seq to measure transcript abundance in 15 Saccharomyces cerevisiae strains from a diverse range of genetic lineages when growing in rich media (YPD) to characterize differential expression across strains.
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| Overall design |
RNA-seq and transcriptome analysis of BY4743 and 14 wild S. cerevisiae isolates. Three biological replicate cell samples (collected on different days) were harvested for RNASeq analysis. Cells were grown in YPD for at least three generations to log phase (OD600 ~0.5) and collected by centrifugation.
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| Contributor(s) |
Gasch AP, Robinson D |
| Citation(s) |
34338637 |
| Submission date |
Apr 06, 2021 |
| Last update date |
Aug 18, 2021 |
| Contact name |
Audrey Gasch |
| E-mail(s) |
agasch@wisc.edu
|
| Organization name |
University of Wisconsin
|
| Department |
Genetics
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| Street address |
425G Henry mall
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| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53706 |
| Country |
USA |
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| Platforms (1) |
| GPL17342 |
Illumina HiSeq 2500 (Saccharomyces cerevisiae) |
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| Samples (45)
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| This SubSeries is part of SuperSeries: |
| GSE171587 |
Natural Variation in the Fitness Consequences of Gene Amplification in Wild Saccharomyces cerevisiae Isolates |
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| Relations |
| BioProject |
PRJNA720135 |
| SRA |
SRP313695 |
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