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| Status |
Public on May 12, 2024 |
| Title |
Therapeutic efficacy of antimalarial drugs targeting DosRS signaling in Mycobacterium abscessus |
| Organism |
Mycobacteroides abscessus ATCC 19977 |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Regulation of M. abscessus gene expression in WT or dosR mutant and treated with artemisinin
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| |
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| Overall design |
WT or DosR deletion mutant were cultured in 16 mL of Dubos browth +10% ADS and 0.05% Tween 80 starting at an OD of 0.05 in a T-25 flask (in horizontal position). The cultures were treated with 20 uM artemisinin or a DMSO control for 24 hours, RNA was extracted, llibraries generated, sequenced and differential gene expression analyzed.
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| |
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| Contributor(s) |
Belardinelli JM, Williams JT, Johnson BK, Abramovitch RB, Jackson M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
May 12, 2021 |
| Last update date |
May 12, 2024 |
| Contact name |
Robert B. Abramovitch |
| Organization name |
Michigan State University
|
| Department |
Microbiology and Molecular Genetics
|
| Street address |
567 Wilson Road
|
| City |
East Lansing |
| State/province |
MI |
| ZIP/Postal code |
48824 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL30122 |
Illumina HiSeq 2500 (Mycobacteroides abscessus ATCC 19977) |
|
| Samples (8)
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| Relations |
| BioProject |
PRJNA729413 |
| SRA |
SRP319430 |
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