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| Status |
Public on Aug 06, 2021 |
| Title |
Antibiotics exposure impacts the gut microbiota and influences energy and metabolic homeostasis in Spodoptera frugiperda |
| Organisms |
Spodoptera frugiperda; insect gut metagenome |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
Insect gut microbiota plays important roles in acquiring nutrition, preventing pathogens infection, immune responses, and communicating with the environment. Gut microbiota can be affected by some external factors such as foods, temperature, and antibiotics. Spodoptera frugiperda (Lepidoptera: Noctuidae) is an important destructive pest of grain crops all over the world. The function of gut microbiota in S. frugiperda remains to be investigated. In this study, we fed the S. frugiperda with the antibiotic mixture (penicillin, gentamicin, rifampicin, and streptomycin) to perturb the gut microbiota, and further examined the effect of dysbiosis in gut microbiota on the gene expression of S. frugiperda by RNA sequencing. We found the composition and diversity of the gut bacterial community were changed in S. frugiperda after antibiotics treatmen, and the expression of genes related to energy and metabolic process were affected after antibiotics exposure in S. frugiperda. Our work will help understand the role of gut microbiota in insects.
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| Overall design |
Spodoptera frugiperda were reared with the artificial diet with or without antibiotics, respectively from the newly hatched larvae to the 3-day-old sixth instar larvae. RNA-seq and 16S rDNA of midguts were performed to study the effects of gut microflora on host transcription.
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| Contributor(s) |
Chen Y, Zhou H, Lai Y, Wang X |
| Citation(s) |
34659154 |
| Submission date |
May 24, 2021 |
| Last update date |
Apr 20, 2022 |
| Contact name |
Xiaoyun Wang |
| E-mail(s) |
wang_xiaoyun@gibh.ac.cn
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| Organization name |
Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences
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| Department |
Infection and Immunity
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| Street address |
190 Kaiyuan Avenue, Huangpu
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| City |
Guangzhou |
| State/province |
Guangdong |
| ZIP/Postal code |
510530 |
| Country |
China |
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| Platforms (2) |
| GPL29193 |
Illumina NovaSeq 6000 (Spodoptera frugiperda) |
| GPL30191 |
Illumina NovaSeq 6000 (insect gut metagenome; Spodoptera frugiperda) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA732454 |
| SRA |
SRP321194 |
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