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Series GSE178150 Query DataSets for GSE178150
Status Public on Jul 08, 2022
Title Construction the larval and adult zebrafish cell atlas at single cell resolution
Organism Danio rerio
Experiment type Expression profiling by high throughput sequencing
Summary Single-cell mRNA sequencing (scRNA-seq) technologies are reshaping the current cell-type classification system. In previous studies, we built the mouse cell atlas (MCA) and human cell landscape (HCL) to catalog all cell types by collecting scRNA-seq data. However, systematically study for zebrafish (Danio rerio) and fruit fly (Drosophila melanogaster) are still lacking. Here, we construct the zebrafish and Drosophila cell atlas with Microwell-seq protocols, which provides valuable resources for characterization of diverse cell populations of zebrafish and Drosophila, and studying difference between vertebrates and Invertebrates at single cell level.
 
Overall design Over 550,000 cells derived from zebrafish whole-body single-cell dissociation and profiling at the larval stage (72 hpf, 3 replicates, 10 fish for each replicate) and adult (90 day, 6 replicates, 1 fish for each replicate).
 
Contributor(s) Guo G, Han X, Wang R, Li J, Wang J, Zhang P
Citation(s) 36229673
Submission date Jun 14, 2021
Last update date Oct 17, 2022
Contact name Guoji Guo
Organization name Center for Stem Cell and Regenerative Medicine
Department Zhejiang University School of Medicine
Street address Yuhangtang street 866
City Hanzhou
ZIP/Postal code 310058
Country China
 
Platforms (2)
GPL23085 HiSeq X Ten (Danio rerio)
GPL30277 DNBSEQ-T7 (Danio rerio)
Samples (106)
GSM5380997 Microwell-seq datasets of Zebrafish_3m_COL3
GSM5380998 Microwell-seq datasets of Zebrafish_3m_COL4
GSM5380999 Microwell-seq datasets of Zebrafish_3m_COL13
This SubSeries is part of SuperSeries:
GSE178151 Construction of the whole animal at a single cell resolution
Relations
BioProject PRJNA737474
SRA SRP324000

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE178150_RAW.tar 1.1 Gb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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