|
| Status |
Public on Nov 08, 2021 |
| Title |
CLIP-seq analysis of KSHV ORF57-associated RNAs |
| Organism |
Homo sapiens |
| Experiment type |
Other
|
| Summary |
Kaposi's sarcoma-associated herpesvirus (KSHV) ORF57 is a viral RNA-binding protein essential for viral lytic gene expression. ORF57 binds to target RNA directly via interaction with cellular cofactors. To investigate the entire repertoire of ORF57-associated RNAs we performed UV cross-linking immunoprecipitatin (CLIP) experiment using an affinity-purified, highly specific anti-ORF57 antibody in KSHV-infected primariy effusion lymphoma BCBL-1 cells undegoing lytic virus replication.
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| |
|
| Overall design |
Cross-linking Immunoprecipitation (CLIP) using antibody against KSHV ORF57 followed by RNA sequencing
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| |
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| Contributor(s) |
Alvarado Hernandez B, Zheng Z, Majerciak V |
| Citation(s) |
34787459, 38410462 |
| Submission date |
Jul 08, 2021 |
| Last update date |
Mar 07, 2024 |
| Contact name |
Zhi-Ming Zheng |
| Organization name |
National Cancer Institute
|
| Department |
Center for Cancer Research
|
| Lab |
RNA Biology Laboratory
|
| Street address |
Bldg. 535 Rm. 312
|
| City |
Frederick |
| State/province |
Maryland |
| ZIP/Postal code |
21702-1201 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
|
| Samples (3) |
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| This SubSeries is part of SuperSeries: |
| GSE179742 |
Protein-RNA interactome analysis of KSHV ORF57 in BCBL-1 cells with lytic virus infection |
|
| Relations |
| BioProject |
PRJNA744779 |
| SRA |
SRP327489 |
