|
| Status |
Public on Sep 27, 2021 |
| Title |
Differential gene regulation in DAPT-treated Hydra reveals candidate direct Notch signalling targets |
| Organism |
Hydra vulgaris |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In Hydra, Notch inhibition causes defects in head patterning and prevents differentiation of proliferating nematocyte progenitor cells into mature nematocytes. To understand the molecular mechanisms by which the Notch pathway regulates these processes, we performed RNA-seq and identified genes that are differentially regulated in response to 48 h of treating the animals with the Notch inhibitor DAPT. To identify candidate direct regulators of Notch signalling, we profiled gene expression changes that occur during subsequent restoration of Notch activity and performed promoter analyses to identify RBPJ transcription factor-binding sites in the regulatory regions of Notch-responsive genes. Interrogating the available single-cell sequencing data set revealed the gene expression patterns of Notch-regulated Hydra genes. Through these analyses, a comprehensive picture of the molecular pathways regulated by Notch signalling in head patterning and in interstitial cell differentiation in Hydra emerged. As prime candidates for direct Notch target genes, in addition to Hydra (Hy)Hes, we suggest Sp5 and HyAlx. They rapidly recovered their expression levels after DAPT removal and possess Notch-responsive RBPJ transcription factorbinding sites in their regulatory regions
|
| |
|
| Overall design |
We aimed to identify the transcriptional target genes of Notch signalling by comparing DAPT-treated animals to control ones at three different time points. Six biological repeats were used.
|
| |
|
| Contributor(s) |
Moneer J, Boettger A |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Sep 20, 2021 |
| Last update date |
Sep 27, 2021 |
| Contact name |
Jasmin Moneer |
| Organization name |
LMU
|
| Department |
Biologie II
|
| Lab |
Boettger
|
| Street address |
Grosshadernerstrasse
|
| City |
Planegg-Martinsried |
| State/province |
Munich |
| ZIP/Postal code |
82152 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL27013 |
Illumina HiSeq 2000 (Hydra vulgaris) |
|
| Samples (36)
|
|
| Relations |
| BioProject |
PRJNA764766 |
| SRA |
SRP337977 |
Less...
More...