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| Status |
Public on Nov 08, 2022 |
| Title |
The single-cell landscape reveals kidney immune cell transcriptional heterogeneity in early diabetic kidney disease |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The pathogenesis of diabetic kidney disease (DKD) involves multifactorial processes that converge to initiate and advance the disease. Although DKD is not typically classified an inflammatory glomerular disease, mounting evidence supports the involvement of renal inflammation as a key contributor in DKD pathogenesis. However, detailed characteristics of specific immune cell types in the context of diabetic kidneys remain poorly understood. To capture the gene expression changes in specific immune cell subsets in early DKD, we performed a single-cell transcriptomic analysis of CD45-enriched kidney immune cells from control and diabetic OVE26 mice. Unsupervised clustering identified 11 distinct cell types that represented the major immune cells and an unidentified cell type. Further analysis of mononuclear phagocytes showed increased macrophage subsets in the diabetic kidneys and that pro-inflammatory and anti-inflammatory macrophages were concomitantly increased. Moreover, rather than in discrete M1 or M2 states, many macrophage subsets expressed genes consistent with the continuum of activation and differentiation states, and that their gene expression shifted toward increased inflammatory and differentiated status in the diabetic kidneys. Our study provides a comprehensive analysis of immune cell transcriptomic profiles in mouse DKD and underscores the dynamic macrophage activation in promoting DKD.
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| Overall design |
Single-cell transcriptomic analysis of CD45-enriched kidney immune cells from control and diabetic OVE26 mice.
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| Contributor(s) |
Fu J, Sun Z, Wang X, Zhang T, Yuan W, Zhang W, Lee K, He JC |
| Citation(s) |
36108806 |
| Submission date |
Jan 31, 2022 |
| Last update date |
Nov 08, 2022 |
| Contact name |
Zeguo Sun |
| E-mail(s) |
zeguo.sun@mssm.edu
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| Phone |
6466427380
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| Organization name |
Ichan School of Medicine at Mount Sinai
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| Street address |
1468 Madison Avenue
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| City |
New York |
| ZIP/Postal code |
10029 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA802200 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE195799_Expr_CD45.txt.gz |
29.3 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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